Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

被引:70
作者
Packard, ER
Parton, R
Coote, JG
Fry, NK
机构
[1] Hlth Protect Agcy, Resp & System Infect Lab, Specialist & Reference Microbiol Div, London NW9 5HT, England
[2] Univ Glasgow, Inst Biomed & Life Sci, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland
关键词
D O I
10.1099/jmm.0.05515-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To determine the value of gene markers for surveillance and to assess the genetic stability of potential acellular pertussis vaccine components, the sequence variation in ten virulence-related genes of Bordetella pertussis was investigated in strains isolated in the UK between 1920 and 2002. These genes encode: pertactin (prnA); pertussis toxin subunits Si (ptxA) and S3 (ptxC); tracheal colonization factor (tcfA); bordetella autotransporter protein C (bapC); bordetella resistance to killing protein (brkA); fimbrial antigen 2 (fim2); outer-membrane protein Q (ompQ); virulence-activated gene 8 (vag8) and adenylate cyclase toxin (cyaA). The encoded proteins are either components of current acellular vaccines (ACVs), or potential virulence markers for B. pertussis. Three strains used in the pertussis UK whole-cell vaccine (WCV), strain Tohama-I used for production of ACV components and the type strain of B. pertussis (18323 T) were also analysed. Several novel alleles were found. The UK isolates were assigned multi-locus sequence types (MLSTs) according to a previously described scheme for B. pertussis based on three of these genes (ptxA, ptxC and tcfA). Compared with isolates from other countries, the UK clinical strains showed a distinct distribution of MLSTs. Apart from one strain that was MLST-3, all other recent isolates (2000-2002) were identified as MLST-5. These isolates differed from the three WCV strains, which were MLST-2 or MLST-3, the Tohama-I strain (MLST-2) and the type strain of B. pertussis (MLST-9). MLST-3 and MLST-5 differ only by a single synonymous mutation, but this method does indicate that currently circulating strains of B. pertussis are not identical to the vaccine types, and they may differ in other important characteristics. Two new MLSTs were identified amongst historical UK isolates. Sequence-based typing offers a convenient method of analysing and comparing populations of B. pertussis from different time periods and from different countries. The variation exhibited by prnA and fim2 suggests that they could be useful, additional epidemiological markers in such a typing scheme.
引用
收藏
页码:355 / 365
页数:11
相关论文
共 31 条
  • [1] THE C-TERMINAL DOMAIN IS ESSENTIAL FOR PROTECTIVE ACTIVITY OF THE BORDETELLA-PERTUSSIS ADENYLATE CYCLASE-HEMOLYSIN
    BETSOU, F
    SEBO, P
    GUISO, N
    [J]. INFECTION AND IMMUNITY, 1995, 63 (09) : 3309 - 3315
  • [2] CLONING AND SEQUENCE OF THE BORDETELLA-BRONCHISEPTICA ADENYLATE CYCLASE-HEMOLYSIN-ENCODING GENE - COMPARISON WITH THE BORDETELLA-PERTUSSIS GENE
    BETSOU, F
    SISMEIRO, O
    DANCHIN, A
    GUISO, N
    [J]. GENE, 1995, 162 (01) : 165 - 166
  • [3] BLACKBURN P, 2000, THESIS U GLASGOW UK
  • [4] Intranasal murine model of Bordetella pertussis infection:: II.: Sequence variation and protection induced by a tricomponent acellular vaccine
    Boursaux-Eude, C
    Thiberge, S
    Carletti, G
    Guiso, N
    [J]. VACCINE, 1999, 17 (20-21) : 2651 - 2660
  • [5] Recommendations are needed for adolescent and adult pertussis immunisation:: rationale and strategies for consideration
    Campins-Martí, M
    Cheng, HK
    Forsyth, K
    Guiso, N
    Halperin, S
    Huang, LM
    Mertsola, J
    Oselka, G
    Ward, J
    von König, CHW
    Zepp, F
    [J]. VACCINE, 2001, 20 (5-6) : 641 - 646
  • [6] Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999
    Cassiday, P
    Sanden, G
    Heuvelman, K
    Mooi, F
    Bisgard, KM
    Popovic, T
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2000, 182 (05) : 1402 - 1408
  • [7] Severe and unrecognised: pertussis in UK infants
    Crowcroft, NS
    Booy, R
    Harrison, T
    Spicer, L
    Britto, J
    Mok, Q
    Heath, P
    Murdoch, I
    Zambon, M
    George, R
    Miller, E
    [J]. ARCHIVES OF DISEASE IN CHILDHOOD, 2003, 88 (09) : 802 - 806
  • [8] TRACHEAL COLONIZATION FACTOR - A BOUDETELLA-PERTUSSIS SECRETED VIRULENCE DETERMINANT
    FINN, TM
    STEVENS, LA
    [J]. MOLECULAR MICROBIOLOGY, 1995, 16 (04) : 625 - 634
  • [9] Genotypic variation in the Bordetella pertussis virulence factors pertactin and pertussis toxin in historical and recent clinical isolates in the United Kingdom
    Fry, NK
    Neal, S
    Harrison, TG
    Miller, E
    Matthews, R
    George, RC
    [J]. INFECTION AND IMMUNITY, 2001, 69 (09) : 5520 - 5528
  • [10] Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation
    Gaia, V
    Fry, NK
    Harrison, TG
    Peduzzi, R
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) : 2932 - 2939