Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe

被引：41

作者：

Ohi, R ^{[1
]}

Feoktistova, A ^{[1
]}

Gould, KL ^{[1
]}

机构：

[1] VANDERBILT UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT CELL BIOL, NASHVILLE, TN 37232 USA

来源：

GENE | 1996年 / 174卷 / 02期

关键词：

fission yeast; plasmids; cloning; gene disruption; recombinant DNA;

D O I：

10.1016/0378-1119(96)00085-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The construction of vectors for use in Schizosaccharomyces pombe using the his3(+) gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

引用

页码：315 / 318

页数：4

共 7 条

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