The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants

被引:84
作者
Saubidet, MI [1 ]
Fatta, N [1 ]
Barneix, AJ [1 ]
机构
[1] Univ Buenos Aires, Fac Agron, IBYF, CONICET, RA-1417 Buenos Aires, DF, Argentina
关键词
Azospirillum brasilense; inoculation; nitrogen uptake; Triticum aestivum L;
D O I
10.1023/A:1020469603941
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Azospirillium brasilense is a rhizosphere bacteria that has been reported to improve yield when inoculated on wheat plants. However, the mechanisms through which this effect is induced is still unclear. In the present work, we have studied the effects of inoculating a highly efficient A. brasilense strain on wheat plant grown in 5 kg pots with soil in a greenhouse, under three N regimes (0, 3 or 16 mM NO3-, 50 ml/pot once or twice-a-week),and in disinfected or non-disinfected soil. At the booting stage, the inoculated roots in both soils showed a similar colonization by Azospirillum sp. that was not affected by N addition. The plants grown in the disinfected soil showed a higher biomass, N content and N concentration than those in the non-disinfected soil, and in both soils the inoculation stimulated plant growth, N accumulation, and N and NO3- concentration in the tissues. At maturity, the inoculated plants showed a higher biomass, grain yield and N content than the uninoculated ones in both soils, and a higher grain protein concentration than the uninoculated. It is concluded that in the present experiments, A. brasilense increased plant growth by stimulating nitrogen uptake by the roots.
引用
收藏
页码:215 / 222
页数:8
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