Preparation and evaluation of a novel monolithic column containing double octadecyl chains for reverse-phase micro high performance liquid chromatography

被引：18

作者：

Duan, Qianqian ^{[1
,2
]}

Liu, Chusheng ^{[1
,2
]}

Liu, Zhenghua ^{[1
,2
]}

Zhou, Zhengyin ^{[1
,2
]}

Chen, Weijia ^{[1
,2
]}

Wang, Qiqin ^{[1
,2
]}

Crommen, Jacques ^{[1
,2
,3
]}

Jiang, Zhengjin ^{[1
,2
]}

机构：

[1] Jinan Univ, Dept Pharm, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Tradit Chine, Guangzhou 510632, Guangdong, Peoples R China

[3] Univ Liege, Dept Pharmaceut Sci, Lab Analyt Pharmaceut Chem, B-4000 Liege, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2014年 / 1345卷

基金：

中国国家自然科学基金;

关键词：

Monoliths; Reverse-phase liquid chromatography; Double alkyl chains; Micro-HPLC; STEARYL-ACRYLATE MONOLITHS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IONIZATION MASS-SPECTROMETRY; CAPILLARY ELECTROCHROMATOGRAPHY; STATIONARY PHASES; POLYMERIC MONOLITHS; TOCOPHEROL ISOMERS; CONTINUOUS BEDS; VITAMIN-E; SEPARATION;

D O I：

10.1016/j.chroma.2014.04.036

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A novel reverse-phase monolithic stationary phase containing double long alkyl chains was prepared based on the thermally initiated co-polymerization of 3-methylacryloyl-3-oxapropyl-3-(N,N-dioctadecylcarbamoyl)-propionate (AOD) and ethylene glycol dimethacrylate (EDMA) in the presence of 2-methyl-1-propanol and 1,4-butanediol as the selected porogens. The polymerization was carefully optimized and good permeability, stability and column efficiency were observed for the final monolithic columns. The column also showed good long term stability and reproducibility. The methylene selectivity demonstrates typical reversed phase characteristics. The optimized poly (AOD-co-EDMA) monolith exhibited good selectivity for a range of non-polar test analytes such as PAHs, tocopherols and alkylphenones. A good separation of intact proteins was also observed. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：174 / 181

页数：8

共 59 条

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