A binary DNA probe for highly specific nucleic acid recognition

被引：106

作者：

Kolpashchikov, Dmitry M. ^{[1
]}

机构：

[1] Columbia Univ, Dept Med, Div Expt Therapeut, New York, NY 10032 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 32期

关键词：

MOLECULAR BEACONS; DISCRIMINATION; HYBRIDIZATION; AFFINITY;

D O I：

10.1021/ja0628093

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new concept for nucleic acid probe design is reported. The extremely high selectivity of the probe is predetermined by cooperative hybridization of the two relatively short ( 10 nucleotide) DNA hairpin fragments to the analyte. A binary DNA probe fluorescently reports the presence of 0.5% of the analyte in excess amount of a single base substituted oligodeoxyribonucleotide and distinguishes single nucleotide substitutions at any position of a 20-mer oligonucleotide at room temperature.

引用

页码：10625 / 10628

页数：4

共 13 条

[1] Target-assembled tandem oligonucleotide systems based on exciplexes for detecting DNA mismatches and single nucleotide polymorphisms [J].

Bichenkova, EV ;

Savage, HE ;

Sardarian, AR ;

Douglas, KT .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 332 (04) :956-964

[2] Thermodynamic basis of the enhanced specificity of structured DNA probes [J].

Bonnet, G ;

Tyagi, S ;

Libchaber, A ;

Kramer, FR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6171-6176

[3] Two sides of the coin: affinity and specificity of nucleic acid interactions [J].

Demidov, VV ;

Frank-Kamenetskii, MD .

TRENDS IN BIOCHEMICAL SCIENCES, 2004, 29 (02) :62-71

[4] Binary malachite green aptamer for fluorescent detection of nucleic acids [J].

Kolpashchikov, DM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (36) :12442-12443

[5] Superselective labelling of proteins: Approaches and techniques [J].

Kolpashchikov, DM .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2003, 21 (01) :55-64

[6] Sensitized photomodification of mammalian DNA polymerase β.: A new approach for highly selective affinity labeling of polymerases [J].

Kolpashchikov, DM ;

Rechkunova, NI ;

Dobrikov, MI ;

Khodyreva, SN ;

Lebedeva, NA ;

Lavrik, OI .

FEBS LETTERS, 1999, 448 (01) :141-144

[7] Structures of helical junctions in nucleic acids [J].

Lilley, DMJ .

QUARTERLY REVIEWS OF BIOPHYSICS, 2000, 33 (02) :109-159

[8] PCR-based detection of minority point mutations [J].

Makrigiorgos, GM .

HUMAN MUTATION, 2004, 23 (05) :406-412

[9] Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes [J].

Marras, SAE ;

Tyagi, S ;

Kramer, FR .

CLINICA CHIMICA ACTA, 2006, 363 (1-2) :48-60

[10] Target discrimination by surface-immobilized molecular beacons designed to detect Francisella tularensis [J].

Ramachandran, A ;

Flinchbaugh, J ;

Ayoubi, P ;

Olah, GA ;

Malayer, JR .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (07) :727-736

← 1 2 →