Benzidine-DNA adduct levels in human peripheral white blood cells significantly correlate with levels in exfoliated urothelial cells

被引:30
作者
Zhou, Q
Talaska, G
Jaeger, M
Bhatnagar, VK
Hayes, RB
Zenzer, TV
Kashyap, SK
Lakshmi, VM
Kashyap, R
Dosemeci, M
Hsu, FF
Parikh, DJ
Davis, B
Rothman, N
机构
[1] UNIV CINCINNATI,DEPT ENVIRONM HLTH,CINCINNATI,OH 45267
[2] NATL INST OCCUPAT HLTH,AHMEDABAD,GUJARAT,INDIA
[3] NCI,DIV CANC EPIDEMIOL & GENET,BETHESDA,MD 20892
[4] VET ADM MED CTR,ST LOUIS,MO 63125
[5] ST LOUIS UNIV,MED CTR,ST LOUIS,MO 63125
关键词
white blood cells; exfoliated urothelial cells; benzidine; DNA adduct; biomonitoring;
D O I
10.1016/S1383-5718(97)00097-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In a cross-sectional study of 33 workers exposed to benzidine and benzidine dyes and 15 non-exposed controls, we previously reported that exposure status and internal dose of benzidine metabolites were strongly correlated with the levels of specific benzidine-DNA adducts in exfoliated urothelial cells. We also evaluated DNA adduct levels in peripheral white blood cells (WBC) of a subset of Is exposed workers and 7 controls selected to represent a wide range of adducts in exfoliated urothelial cells. Samples were coded and then DNA was analyzed using P-32-postlabeling, along with n-butanol extraction. One adduct, which co-chromatographed with a synthetic N-(3'-phospho-deoxyguanosin-8-yl)-N'-acetylbenzidine standard, predominated in those samples with adducts present. The median level (range) of this adduct in WBC DNA was 194.4 (3.2-975) RAL X 10(9) in exposed workers and 1.4 (0.1-6.4) in the control subjects (p = 0.0002, Wilcoxon Rank Sum Test). There was a striking correlation between WBC and exfoliated urothelial cell adduct levels (Pearson r = 0.84, p < 0.001) among exposed subjects. In addition, the sum of urinary benzidine, N-acetylbenzidine and N,N'-diacetylbenzidine correlated with the levels of this adduct in both tissues. This is the first study in humans to show a relationship for a specific carcinogen adduct in a surrogate tissue and in urothelial cells, the target for urinary bladder cancer. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:199 / 205
页数:7
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