HIV-1 integration sites are flanked by potential MARs that alone can act as promoters

被引：21

作者：

Kulkarni, A ^{[1
]}

Pavithra, L ^{[1
]}

Rampalli, S ^{[1
]}

Mogare, D ^{[1
]}

Babu, K ^{[1
]}

Shiekh, G ^{[1
]}

Ghosh, S ^{[1
]}

Chattopadhyay, S ^{[1
]}

机构：

[1] Natl Ctr Cell Sci, Pune 411007, Maharashtra, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 322卷 / 02期

关键词：

HIV-1; integration; matrix associated regions; IgH MAR; p53; promoter; human genome analysis;

D O I：

10.1016/j.bbrc.2004.07.170

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix attachment regions (MARs) are cis regulatory elements that modulate gene expression in a tissue and cell stage specific manner. Recent reports show that viral integration within the genome takes place at nonrandom active genes. We have checked for the presence of MARs in the vicinity of the reported 524 HIV-1 integration sites. Our studies show that in 92.5% cases, MARs flank the integration sites. Similarly, for adeno-associated virus, two potential MARs were present next to the integration site on the human chromosome. Earlier we have shown that short MAR sequences present upstream of HIV-1 LTR promote processive transcription at a distance. Here, using a well-studied IgH-MAR and another potential MAR from p53 promoter, we demonstrate that MARs alone can act as promoters. Thus, we propose that MAR elements near the HIV-1 integration sites can act as potential promoters, which may facilitate proviral integration and transcription. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：672 / 677

页数：6

共 27 条

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