Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function

被引：69

作者：

Jaw, TJ

You, LR

Knoepfler, PS

Yao, LC

Pai, CY

Tang, CY

Chang, LP

Berthelsen, J

Blasi, F

Kamps, MP

Sun, YH ^{[1
]}

机构：

[1] Natl Yang Ming Univ, Inst Genet, Taipei 112, Taiwan

[2] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan

[3] Univ Calif San Diego, Sch Med, Dept Pathol, La Jolla, CA 92093 USA

[4] HS Raffaele, DIBIT, Mol Genet Unit, I-20131 Milan, Italy

来源：

MECHANISMS OF DEVELOPMENT | 2000年 / 91卷 / 1-2期

关键词：

Drosophila; Homothorax; Extradenticle; PREP1; nuclear export; HOX; eye; antenna;

D O I：

10.1016/S0925-4773(99)00316-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH, This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：279 / 291

页数：13

共 43 条

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Abu-Shaar M, 1998, DEVELOPMENT, V125, P3821

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