Rapid detection of polymorphisms of the nitric oxide cascade

被引：6

作者：

Weiss, J ^{[1
]}

Fricker, R ^{[1
]}

Haefeli, WE ^{[1
]}

机构：

[1] Univ Heidelberg, Dept Internal Med 4, D-69115 Heidelberg, Germany

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2002年 / 40卷 / 04期

关键词：

nitric oxide synthase (NOS3); NAD(P)H oxidoreductases (p22phox); polymerase chain reaction (PCR); LightCycler (TM); hybridization probes;

D O I：

10.1515/CCLM.2002.054

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

NOS3 (endothelial nitric oxide (NO) synthase) and p22phox (subunit of NAD(P)H oxidase) are two genes whose products are involved in formation and degradation of NO, a ubiquitous signaling molecule largely responsible for the maintenance of normal endothelial function. The G894T polymorphism of NOS3 and the C242T polymorphism of p22phox are reportedly associated with numerous cardiovascular diseases. For each polymorphism we developed a rapid and reliable method with the hybridization probes format on the LightCycler(TM) and compared it with conventional PCR-restriction fragment length polymorphism (PCR-RFLP) analysis with regard to reliability, duration and cost. The new methods are more reliable, faster and less expensive than PCR-RFLP analysis and therefore represent a significant advantage in the detection of two candidate risk factors for cardiovascular diseases.

引用

页码：341 / 344

页数：4

共 22 条

[1] NADPH oxidase: An update [J].