Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

被引:21
作者
Adachi, Sumiko [1 ]
Nobusawa, Takashi [1 ]
Umeda, Masaaki [1 ]
机构
[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan
基金
日本学术振兴会;
关键词
Arabidopsis; Cyclin-dependent kinase; Cell division; Epidermis; Gene expression; EXPRESSION; DIVISION; GENES; PROLIFERATION; PROTEIN; CDC2A; DIFFERENTIATION; ACTIVATION; PROMOTER; MUTANTS;
D O I
10.1016/j.ydbio.2009.03.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A-type cyclin-dependent kinase (CDKA) is an ortholog of yeast Cdc2/Cdc28p, and is assumed to have an essential function in plant growth and organogenesis. Previous studies revealed that its kinase activity is controlled by post-translational modifications, such as binding to cyclins and phosphorylations, but its transcriptional regulation is poorly understood. Here, we generated a promoter dissection series of Arabidopsis (Arabidopsis thaliana) CDKA;I, and used beta-glucuronidase (GUS) gene-fused reporter constructs for expression analyses in planta. The results revealed two types of transcriptional control in shoots: general quantitative regulation and cell type-specific regulation. We identified a promoter region that promotes CDKA;1 expression in the leaf epidermis, but not in the L1 layer of the shoot apical meristem. This region also directed abaxial side-biased expression, which may be linked to the adaxial/abaxial side specification. Another reporter construct showed that CDKA; I expression in the inner layers of leaves is controlled by a distinct regulatory region in the promoter. These results suggest that the transcriptional regulation of CDKA; I may play a key role in proper development of leaves by coordinating cell division and differentiation of different cell types. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:306 / 314
页数:9
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