Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes

被引：40

作者：

Hougaard, DM

Hansen, H

Larsson, LI

机构：

[1] Department of Molecular Cell Biology, Statens Serum Institut, Building 81, DK-2300 Copenhagen S

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 1997年 / 108卷 / 4-5期

关键词：

D O I：

10.1007/s004180050174

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Synthetic oligodeoxynucleotides represent attractive tools for mRNA in situ hybridization. In the present review, guidelines for the preparation, labeling and use of such probes is discussed. Emphasis is placed on sensitivity and specificity issues.

引用

页码：335 / 344

页数：10

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