A luminescence endotoxin biosensor prepared by the sol-gel method

被引：16

作者：

Hreniak, A ^{[1
]}

Maruszewski, K

Rybka, J

Gamian, A

Czyzewski, J

机构：

[1] Wroclaw Tech Univ, Fac Mech Engn, PL-50370 Wroclaw, Poland

[2] Polish Acad Sci, Inst Low Temp & Struct Res, PL-50950 Wroclaw, Poland

[3] Polish Acad Sci, Inst Immunol & Expt Therapy, PL-50422 Wroclaw, Poland

[4] ABB Corp Res, PL-31038 Krakow, Poland

来源：

OPTICAL MATERIALS | 2004年 / 26卷 / 02期

关键词：

thin films; sol-gel process; luminescence depolarization; antibodies detection;

D O I：

10.1016/j.optmat.2003.11.013

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Silicate thin films with terminal amino groups were prepared via the sol-gel technique and selected biological molecules labeled with luminophores were covalently attached to their surfaces. The obtained thin films with covalently bound Concanavalin-A (ConA) labeled with fluorescein (FITC) were immersed in a buffer and polarized emission spectra were measured. In the next step, the samples were incubated with a lipopolysaccharide (LPS) isolated from Hafnia alvei PCM 1186 bacterial strain and the emission spectra were collected again. The influence of LPS binding to Con-A on the luminescence depolarization has been proven. The system based on the this effect can be employed as an optical sensor for detection of certain types of bacteria. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：141 / 144

页数：4

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