Isotopic evidence of full and partial myco-heterotrophy in the plant tribe Pyroleae (Ericaceae)

被引:59
作者
Hynson, Nicole A. [1 ]
Preiss, Katja [2 ]
Gebauer, Gerhard [2 ]
Bruns, Thomas D. [3 ]
机构
[1] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA
[2] Univ Bayreuth, BayCEER, Biogeochim Isotop Lab, Bayreuth, Germany
[3] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
关键词
(15)N and (13)C; Ericaceae; mixotrophy; myco-heterotrophy; mycorrhiza; Pyroleae; Pyrola; pyroloids; NATURAL-ABUNDANCE; NONPHOTOSYNTHETIC INDIVIDUALS; CARBON ISOTOPES; NITROGEN; N-15; ECTOMYCORRHIZAL; ORCHIDS; FUNGI; C-13; DISCRIMINATION;
D O I
10.1111/j.1469-8137.2009.02781.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Botanists and mycologists have long debated the potential for full myco-heterotrophy in the achlorophyllous Pyrola aphylla (Ericaceae). Here we address the ecophysiology of this putative myco-heterotroph and two other closely related green species in the tribe Pyroleae (Pyrola picta and Chimaphila umbellata). The stable isotopes of carbon and nitrogen (delta(13)C and delta(15)N) were analysed from 10 populations of Pyroleae species in California and Oregon, USA. For all populations isotope signatures were tested for significant differences between P. aphylla, green pyroloids, surrounding autotrophs and obligate myco-heterotrophs. Throughout all populations P. aphylla was most similar to myco-heterotrophs that associate with ectomycorrhizal fungi in its (13)C signature (average enrichment epsilon(13)C = 6.9 +/- 0.9 parts per thousand) and even more enriched in (15)N than many previously recorded myco-heterotrophic species (average enrichment epsilon(15)N = 18.0 +/- 2.2 parts per thousand). The two green Pyroleae species were not enriched in (13)C compared with the autotrophic understory (C. umbellata average enrichment epsilon(13)C = -0.5 +/- 1.0 parts per thousand and P. picta average epsilon(13)C = 0.3 +/- 1.4 parts per thousand) and their (15)N signatures were similar to myco-heterotrophs that associate with ectomycorrhizal fungi (C. umbellata average enrichment epsilon(15)N = 10.6 +/- 1.6 parts per thousand and P. picta average epsilon(15)N = 10.6 +/- 1.9 parts per thousand). This is the first study to analyse the isotope signatures of P. aphylla from a wide geographic region and our results confirm the variable trophic strategies of adult plants within the Pyroleae and the myco-heterotrophic status of P. aphylla. New Phytologist (2009) 182: 719-726doi: 10.1111/j.1469-8137.2009.02781.x.
引用
收藏
页码:719 / 726
页数:8
相关论文
共 38 条
[1]   Cephalanthera longifolia (Neottieae, Orchidaceae) is mixotrophic:: a comparative study between green and nonphotosynthetic individuals [J].
Abadie, Jean-Claude ;
Puttsepp, Ulle ;
Gebauer, Gerhard ;
Faccio, Antonella ;
Bonfante, Paola ;
Selosse, Marc-Andre .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 2006, 84 (09) :1462-1477
[2]   Post-photo synthetic fractionation of stable carbon isotopes between plant organs -: a widespread phenomenon [J].
Badeck, FW ;
Tcherkez, G ;
Nogués, S ;
Piel, C ;
Ghashghaie, J .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (11) :1381-1391
[3]   Changing partners in the dark: isotopic and molecular evidence of ectomycorrhizal liaisons between forest orchids and trees [J].
Bidartondo, MI ;
Burghardt, B ;
Gebauer, G ;
Bruns, TD ;
Read, DJ .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2004, 271 (1550) :1799-1806
[4]   Carbon isotopes in terrestrial ecosystem pools and CO2 fluxes [J].
Bowling, David R. ;
Pataki, Diane E. ;
Randerson, James T. .
NEW PHYTOLOGIST, 2008, 178 (01) :24-40
[5]  
CAMP W. H., 1940, BULL TORREY BOT CLUB, V67, P453, DOI 10.2307/2480967
[6]   Stable isotopes in plant ecology [J].
Dawson, TE ;
Mambelli, S ;
Plamboeck, AH ;
Templer, PH ;
Tu, KP .
ANNUAL REVIEW OF ECOLOGY AND SYSTEMATICS, 2002, 33 :507-559
[7]  
De Jussieu A.L., 1789, Genera plantarum secundum ordines naturales disposita
[8]   Natural abundance of 15N in forests across a nitrogen deposition gradient [J].
Emmett, BA ;
Kjonaas, OJ ;
Gundersen, P ;
Koopmans, C ;
Tietema, A ;
Sleep, D .
FOREST ECOLOGY AND MANAGEMENT, 1998, 101 (1-3) :9-18
[9]   CARBON ISOTOPE DISCRIMINATION AND PHOTOSYNTHESIS [J].
FARQUHAR, GD ;
EHLERINGER, JR ;
HUBICK, KT .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1989, 40 :503-537
[10]   Relationships and character transformation in Pyroloideae (Ericaceae) based on ITS sequences, morphology, and development [J].
Freudenstein, JV .
SYSTEMATIC BOTANY, 1999, 24 (03) :398-408