Modification of mononuclear cell function after incubation with albumin-bound unsaturated fatty acids or soybean oil emulsion

被引:4
作者
Borsum, K
Aksnes, J
Muller, F
Hagve, TA
Kierulf, P
Rollag, H
机构
[1] UNIV OSLO,NATL HOSP,DEPT CLIN BIOCHEM,N-0316 OSLO,NORWAY
[2] UNIV OSLO,NATL HOSP,CARDIOTHORAC UNIT,DEPT SURG,N-0316 OSLO,NORWAY
[3] UNIV OSLO,NATL HOSP,INST MICROBIOL,N-0316 OSLO,NORWAY
[4] UNIV OSLO,ULLEVAL UNIV HOSP,DEPT CLIN CHEM,N-0316 OSLO,NORWAY
关键词
immune response; linoleic acid; lipid emulsion; lymphocyte activity; membrane fluidity; mononuclear cell activity; phospholipids; plasma membrane; unsaturated fatty acids;
D O I
10.1111/j.1699-0463.1997.tb05070.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Administration of total parenteral nutrition (TPN) with soybean oil emulsion leads to a linoleic acid enrichment of the plasma membrane that may explain an tn vivo activation of mononuclear cells (MNC) seen in our previous studies. Fatty acids from the lipid emulsion may have been accessible to MNC after endocytosis of lipid particles, or by direct uptake of fatty acids after lipoprotein lipase hydrolyzation of the emulsion triglycerides. To resemble the incorporation of fatty acids in vivo, we have modified MNC membrane lipid composition by incubation with different albumin-bound unsaturated fatty acids (UFA) or soybean oil emulsion. After incubation with albumin-bound linoleic and oleic acid: the unstimulated release of superoxide anion was unchanged, while zymosan-stimulated release was 140% (n.s) and 112% (p<0.05) and phorbol-myristate-acetate (PMA)-stimulated release 148% (p<0.05) and 124% (p<0.05) of controls, respectively. Incubation with other UFAs or emulsion did not change superoxide anion release. Unstimulated lymphocyte proliferation increased 3 to 13-fold (p<0.05) after incubation with all UFAs compared to controls, while UFA incubation did not change phytohemagglutinin (PHA) or PMA-stimulated proliferation. Unstimulated lymphocyte proliferation was decreased after incubation with emulsion, while PHA/PMA-stimulated proliferation was unchanged. Increase in membrane fluidity was detectable only after incubation with emulsion. The increased reactivity may have been caused by changes in the lipid environment surrounding membrane-bound enzymes important for signal transduction through the plasma membrane.
引用
收藏
页码:671 / 679
页数:9
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