Cytokine profile of myelin basic protein-reactive T cells in multiple sclerosis and healthy individuals

被引:77
作者
Hermans, G
Stinissen, P
Hauben, L
VandenBergLoonen, E
Raus, J
Zhang, JW
机构
[1] LIMBURGS UNIV CTR, B-3590 DIEPENBEEK, BELGIUM
[2] ACAD ZIEKENHUIS, TISSUE TYPING LAB, MAASTRICHT, NETHERLANDS
关键词
D O I
10.1002/ana.410420106
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Myelin basic protein (MBP)-reactive T cells have been implicated in the autoimmune pathogenesis of multiple sclerosis (MS). In this study, rye examined the cytokine profile of 531 primary MBP-reactive T-cell Lines and 72 independently established clones from 32 patients with MS and 18 healthy controls (NS) by using highly sensitive enzyme-linked immunosorbent assays. An increased number of primary T-cell lines producing interferon-gamma (IFN gamma) and/or interleukin-4 (IL-4) in response to MBP were found in patients with MS compared with controls. No distinct Th1 or Th2 subtypes could be demonstrated among the MBP-reactive clones. IL-4 was more frequently observed among MS-derived clones. Clones derived from MS patients produced increased levels of IL-2, IL-4, tumor necrosis factor-alpha (TNF gamma), IFN gamma, and IL-10, but not IL-6, It is interesting that MBP-reactive T cells from MS patients expressing the disease-associated HLA-DRB1*15 allele produced increased quantities of TNF alpha, a cytokine suggested to play an important role in inflammation and demyelination. When challenged with either MBP or a bacterial superantigen, the clones expressed similar levels of the proinflammatory cytokine IFN gamma. Our study suggests a functional difference in T-cell responses To MBP in patients with MS compared with healthy individuals, and provides further insights into the role of MBP-reactive T cells and their cytokine profile in the inflammatory processes of MS.
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页码:18 / 27
页数:10
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