Overexpression of E2F1 in lung and liver metastases of human colon cancer is associated with gene amplification

被引：48

作者：

Iwamoto, M

Banerjee, D

Menon, LG

Jurkiewicz, A

Rao, PH

Kemeny, NE

Fong, YM

Jhanwar, SC

Gorlick, R

Bertino, JR ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Canc Inst New Jersey, Dept Med, New Brunswick, NJ 08903 USA

[2] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Canc Inst New Jersey, Dept Pharmacol, New Brunswick, NJ 08903 USA

[3] Mem Sloan Kettering Canc Ctr, Dept Med, New York, NY 10021 USA

[4] Mem Sloan Kettering Canc Ctr, Dept Surg, New York, NY 10021 USA

[5] Mem Sloan Kettering Canc Ctr, Dept Human Genet, New York, NY 10021 USA

[6] Mem Sloan Kettering Canc Ctr, Dept Pediat, New York, NY 10021 USA

来源：

CANCER BIOLOGY & THERAPY | 2004年 / 3卷 / 04期

关键词：

E2F-1 gene amplification; 20q; colorectal cancer; metastases;

D O I：

10.4161/cbt.3.4.733

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase ITS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

引用

页码：395 / 399

页数：5

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