Catalysis and rational engineering of trans-acting pH6DZ1, an RNA-cleaving and fluorescence-signaling deoxyribozyme with a four-way junction structure

被引：41

作者：

Shen, Yutu

Chiuman, William

Brennan, John D.

Li, Yingfu

机构：

[1] McMaster Univ, Dept Chem, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 09期

关键词：

aptamers; biosensors; cleavage reactions; deoxyribozymes; DNAzyme; fluorescence;

D O I：

10.1002/cbic.200600195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biosensor made of a catalytic DNA. When the RNA moiety (red) in the fluorogenic DNA substrate (purple) is cleaved by the bound catalytic DNA (blue) with a multiduplex structure, a strong fluorescence signal is generated owing to the separation of the fluorophore (F) from the quencher (Q). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：1343 / 1348

页数：6

相关论文

共 50 条

[1] DNAzymes:: From creation in vitro to application in vivo [J].

Achenbach, JC ;

Chiuman, W ;

Cruz, RPG ;

Li, Y .

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2004, 5 (04) :321-336

[2] Effects of 3′-terminal phosphates in RNA produced by ribozyme cleavage [J].

Alam, SL ;

Nelson, CC ;

Felden, B ;

Atkins, JF ;

Gesteland, RF .

RNA, 1998, 4 (06) :607-612

[3] Engineered allosteric ribozymes as biosensor components [J].

Breaker, RR .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :31-39

[4] Method for assigning double-stranded RNA structures [J].

Brown, TS ;

Bevilacqua, PC .

BIOTECHNIQUES, 2005, 38 (03) :368-+

[5] Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites [J].

Cairns, MJ ;

King, A ;

Sun, LQ .

NUCLEIC ACIDS RESEARCH, 2003, 31 (11) :2883-2889

[6] Inhibition of HIV-1 gene expression by novel DNA enzymes targeted to cleave HIV-1 TAR RNA: Potential effectiveness against all HIV-1 isolates [J].

Chakraborti, S ;

Banerjea, AC .

MOLECULAR THERAPY, 2003, 7 (06) :817-826

[7] An autonomous DNA nanomotor powered by a DNA enzyme [J].

Chen, Y ;

Wang, MS ;

Mao, CD .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (27) :3554-3557

[8] Revitalization of six abandoned catalytic DNA species reveals a common three-way junction framework and diverse catalytic cores [J].

Chiuman, W ;

Li, YF .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 357 (03) :748-754

[9] Dinucleotide junction cleavage versatility of 8-17 deoxyribozyme [J].

Cruz, RPG ;

Withers, JB ;

Li, YF .

CHEMISTRY & BIOLOGY, 2004, 11 (01) :57-67

[10] Ribozyme speed limits [J].

Emilsson, GM ;

Nakamura, S ;

Roth, A ;

Breaker, RR .

RNA, 2003, 9 (08) :907-918

← 1 2 3 4 5 →