Direct electroporation of clostridial hydrogenase into cyanobacterial cells

被引：17

作者：

Miyake, M ^{[1
]}

Asada, Y ^{[1
]}

机构：

[1] NATL INST BIOSCI & HUMAN TECHNOL, TSUKUBA, IBARAKI 305, JAPAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 11期

关键词：

D O I：

10.1023/A:1018417023074

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clostridial hydrogenase was introduced into a cyanobacterium, Synechococcus elongatus, by direct electroporation. The introduced hydrogenase activity was 11 nmol H-2 evolved with reduced Methyl Viologen per mg chlorophyll. The cells with clostridial hydrogenase showed simultaneous light-dependent evolution of H-2 and O-2. This method will be applicable to assess a variety of hydrogenases for hydrogen production coupled with cyanobacterial photosynthesis.

引用

页码：787 / 790

页数：4

共 10 条

[1] HYDROGENASE
ADAMS, MWW
MORTENSON, LE
CHEN, JS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 594 (2-3) : 105 - 176
[2] THE PURIFICATION OF HYDROGENASE-II (UPTAKE HYDROGENASE) FROM THE ANAEROBIC N-2-FIXING BACTERIUM CLOSTRIDIUM-PASTEURIANUM
ADAMS, MWW
MORTENSON, LE
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 766 (01) : 51 - 61
[3] SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE-GREEN ALGAE ON PLATES
ALLEN, MM
[J]. JOURNAL OF PHYCOLOGY, 1968, 4 (01) : 1 - &
[4] ASADA Y, 1985, REP FERMENT RES I, V63, P39
[5] Hydrogen biotechnology: Progress and prospects
Benemann, J
[J]. NATURE BIOTECHNOLOGY, 1996, 14 (09) : 1101 - 1103
[6] HYDROGEN EVOLUTION BY A CHLOROPLAST-FERREDOXIN-HYDROGENASE SYSTEM
BENEMANN, JR
BERENSON, JA
KAPLAN, NO
KAMEN, MD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (08) : 2317 - 2320
[7] DEMARSAC NT, 1988, METHOD ENZYMOL, V167, P318
[8] FRY I, 1977, Z NATURFORSCH C, V32, P110
[9] PROLONGED PRODUCTION OF HYDROGEN GAS BY A CHLOROPLAST BIOCATALYTIC SYSTEM
RAO, KK
ROSA, L
HALL, DO
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 68 (01) : 21 - 28
[10] YAMAOKA T, 1978, PLANT CELL PHYSIOL, V19, P943, DOI 10.1093/oxfordjournals.pcp.a075684

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