Polymer surface reorientation after protein adsorption

被引：19

作者：

Clarke, Matthew L. ^{[1
]}

Chen, Zhan ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

LANGMUIR | 2006年 / 22卷 / 21期

关键词：

D O I：

10.1021/la0607656

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

Surface side-chain orientation changes of two polymers have been observed upon protein adsorption using sum frequency generation vibrational spectroscopy. Side-chain-deuterated poly( ethyl methacrylate) and poly(n-butyl methacrylate) were contacted with five protein solutions: albumin, fibrinogen, ubiquitin, cytochrome c, and lysozyme. The CD3/CD2 symmetric stretch ratios of the surface polymer side chains in contact with these different media were compared to each other and to that of the polymer contacting air or phosphate buffered saline. The adsorption of different proteins to the surfaces resulted in polymer side-chain orientations slightly different from each other, with orientations between the air and buffer cases.

引用

收藏

页码：8627 / 8630

页数：4

相关论文

共 37 条

[1]

Rationalizing the design of polymeric biomaterials [J].

Angelova, N ;

Hunkeler, D .

TRENDS IN BIOTECHNOLOGY, 1999, 17 (10) :409-421

[2]

SUM-FREQUENCY VIBRATIONAL SPECTROSCOPY OF THE SOLID-LIQUID INTERFACE [J].

BAIN, CD .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1995, 91 (09) :1281-1296

[3]

DEPTH SENSITIVITY OF WETTING - MONOLAYERS OF OMEGA-MERCAPTO ETHERS ON GOLD [J].

BAIN, CD ;

WHITESIDES, GM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (17) :5897-5898

[4]

Vibrational spectroscopy of interfaces by infrared-visible sum frequency generation [J].

Buck, M ;

Himmelhaus, M .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A, 2001, 19 (06) :2717-2736

[5]

Surface morphology and molecular chemical structure of poly(n-butyl methacrylate)/polystyrene blend studied by atomic force microscopy (AFM) and sum frequency generation (SFG) vibrational spectroscopy [J].

Chen, C ;

Wang, J ;

Woodcock, SE ;

Chen, Z .

LANGMUIR, 2002, 18 (04) :1302-1309

[6]

Comparison of surface structures of poly(ethyl methacrylate) and poly(ethyl acrylate) in different chemical environments [J].

Chen, CY ;

Clarke, ML ;

Wang, J ;

Chen, Z .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2005, 7 (11) :2357-2363

[7]

Sum frequency generation vibrational spectroscopy studies on molecular conformation and orientation of biological molecules at interfaces [J].

Chen, XY ;

Clarke, ML ;

Wang, J ;

Chen, Z .

INTERNATIONAL JOURNAL OF MODERN PHYSICS B, 2005, 19 (04) :691-713

[8]

Studies of polymer surfaces by sum frequency generation vibrational spectroscopy [J].

Chen, Z ;

Shen, YR ;

Somorjai, GA .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2002, 53 :437-465

[9]

Interaction of fibrinogen with surfaces of end-group-modified polyurethanes: A surface-specific sum-frequency-generation vibrational spectroscopy study [J].

Chen, Z ;

Ward, R ;

Tian, Y ;

Malizia, F ;

Gracias, DH ;

Shen, YR ;

Somorjai, GA .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 62 (02) :254-264

[10]

Conformational changes of fibrinogen after adsorption [J].

Clarke, ML ;

Wang, J ;

Chen, Z .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (46) :22027-22035

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