Synergistic mixtures of fungitoxic monochloro and dichloro-8-quinolinols against five fungi

Fourteen mono- and dichloro-8-quinolinols were tested against five fungi ( Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, and Mucor circinelloides) and compared with the fungitoxicity of 8-quinolinol in Yeast Nitrogen Base containing 1% D-glucose and 0.088% L-asparagine. All of the compounds were more fungitoxic than 8-quinolinol except for the surprising activity of 8-quinolinol against A. oryzae. Mixtures of the MICs of monochloro- and dichloro-8-quinolinols in which the halogens were in different positions of the quinoline ring showed synergism. Comparable mixtures in which one position of each compound was occupied by the same halogen showed additive activity. In a different study we showed that 3,5,6-, 3,5,7-, 4,5,7-, and 5,6,7- trichloro-8-quinolinols were not toxic to M. circinelloides, whereas the combinations of the correspondingly substituted mono- and dichloro-8-quinolinols as well as 3,6-dichloro- and 5,7-dichloro-8-quinolinols were inhibitory. This indicated that a steric factor can be involved in affecting fungitoxicity.