Quercetin and anti-CD95(Fas/Apo1) enhance apoptosis in HPB-ALL cell line

被引：76

作者：

Russo, M ^{[1
]}

Palumbo, R ^{[1
]}

Tedesco, I ^{[1
]}

Mazzarella, G ^{[1
]}

Russo, P ^{[1
]}

Iacomino, G ^{[1
]}

Russo, GL ^{[1
]}

机构：

[1] CNR, Inst Food Sci & Technol, I-83100 Avellino, Italy

来源：

FEBS LETTERS | 1999年 / 462卷 / 03期

关键词：

quercetin; CD95/Apo1/Fas; apoptosis; flavonoid; HPB-ALL cell line;

D O I：

10.1016/S0014-5793(99)01544-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several malignant cell lines are resistant to CD95(Apol/Fas)-mediated apoptosis, even when the CD95 receptor is highly expressed. Sensitivity to CD95-1nduced apoptosis can be restored using different molecules. In this study, we showed that quercetin, a naturally occuring flavonoid, in association with the agonistic anti-CD95 monoclonal antibody, increases DNA fragmentation and caspase-3 activity in HPB-ALL cells. These cells have been selected for their known resistance to CD95-induced apoptosis, At molecular level, quercetin lowers the level of intracellular reactive oxygen species, reduces mitochondrial transmembrane potential, thereby leaving the expression of CD95 receptor unchanged. (C) 1999 Federation of European Biochemical Societies.

引用

页码：322 / 328

页数：7

共 54 条

[1] Bcl-2 and the outer mitochondrial membrane in the inactivation of cytochrome c during fas-mediated apoptosis [J].