HPLC-DAD determination of opioids, cocaine and their metabolites in plasma

被引：48

作者：

Fernandez, P. ^{[1
]}

Morales, L. ^{[1
]}

Vazquez, C. ^{[1
]}

Bermejo, A. M. ^{[1
]}

Tabernero, M. J. ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Med, Forens Toxicol Serv, Inst Legal Med, Santiago De Compostela 15782, Spain

来源：

FORENSIC SCIENCE INTERNATIONAL | 2006年 / 161卷 / 01期

关键词：

opioids; cocaine; HPLC-DAD; plasma; solid-phase extraction;

D O I：

10.1016/j.forsciint.2005.10.016

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

High performance liquid chromatography with diode array detection (HPLC-DAD) was used to develop a method for the simultaneous determination of morphine, codeine, 6-acetylmorphine (6AM), cocaine, benzoylecgonine (BEG), cocaethylene, methadone and its metabolite, 2-ethylidene-1,5-dimethyldiphenylpyrrolidine (EDDP), in plasma. Following solid-phase extraction with Bond Elut Certify cartridges, chromatography was performed on an X-Terra((R)) RP8 column (250 mm x 4.6 mm i.d., 5 mu m particle size), using acetonitrile-phosphate buffer pH 6.53 as mobile phase and elution in the gradient mode. The detector response was linear at concentrations over the range 0.1-10 mu g/mL in plasma, and the correlation coefficients for the eight drugs studied were all higher than 0.99. The average extraction recoveries from plasma ranged from 60% for BEG to 95% for methadone. The precision was acceptable, with coefficients of variation oscillating between 2.55% and 6.45%. The accuracy was found to be within satisfactory limits (+/- 8.1%). Finally, the method was applied to 21 plasma samples from fatal overdoses, obtaining positive results for two or more drugs. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：31 / 35

页数：5

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