Intergeneric protoplast fusion between Acinetobacter sp A3 and Pseudomonas putida DP99 for enhanced hydrocarbon degradation

被引:4
作者
Hanson, KG
Desai, AJ
机构
[1] MAHARAJA SAYAJIRAO UNIV BARODA, DEPT MICROBIOL, BARODA 390002, GUJARAT, INDIA
[2] MAHARAJA SAYAJIRAO UNIV BARODA, CTR BIOTECHNOL, BARODA 390002, GUJARAT, INDIA
关键词
D O I
10.1007/BF00129337
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Polyethylene glycol 6000 mediated protoplast fusion between an alkane degrader Acinetobacter sp. A3, and a naphthalene degrader, Pseudomonas putida DP99, resulted in fusants capable of degrading both hydrocarbons and were morphologically similar to Acinetobacter sp. A3. While fusant F4/13 and Pseudomonas putida DP99 degraded over 98% of naphthalene provided by the end of five days, tetradecane degradation by fusant F4/13 was 82% compared to 77% by Acinetobacter sp. A3 in the same time period. Also, while from naphthalene +tetradecane mixture, fusant F4/13 could degrade 99% and 53% of naphthalene and tetradecane respectively, both the parent strains together could degrade over 99% naphthalene but only about 16% tetradecane.
引用
收藏
页码:1369 / 1374
页数:6
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