Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples

被引：86

作者：

Shim, Won-Bo

Yang, Zheng-You

Kim, Ji-Young

Choi, Jin-Gil

Je, Jung-Hyun

Kang, Sung-Jo

Kolosova, Anna Yu

Eremin, Sergei A.

Chung, Duck-Hwa ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Grad Sch, Brain Korea Program 21, Div Appl Life Sci, Chinju 660701, Gyeongnam, South Korea

[2] Univ Ghent, Fac Pharmaceut Sci, Grp Bioanal, Lab Food Anal, B-9000 Ghent, Belgium

[3] Moscow MV Lomonosov State Univ, Fac Chem, Div Chem Enzymol, Moscow 119992, Russia

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 26期

关键词：

atrazine; herbicide; monoclonal antibody; enzyme-linked immunosorbent assay; immunochromatography;

D O I：

10.1021/jf0620057

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10 min to get results and one step of sample to perform the assay. The results of water samples spiked with 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtained by DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screening of atrazine in various water samples.

引用

页码：9728 / 9734

页数：7

共 29 条

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