The Effect of Generation 2 and 3 Poly(amidoamine) Dendrimers on Viability of Human Breast Cancer Cells

被引：31

作者：

Winnicka, Katarzyna ^{[1
]}

Bielawski, Krzysztof ^{[2
]}

Rusak, Magorzata ^{[3
]}

Bielawska, Anna ^{[2
]}

机构：

[1] Med Univ Bialystok, Dept Pharmaceut Technol, Fac Pharm, PL-15089 Bialystok, Poland

[2] Med Univ Bialystok, Dept Med Chem & Drug Technol, Fac Pharm, PL-15089 Bialystok, Poland

[3] Med Univ Bialystok, Dept Hematol Diagnost, Fac Pharm, PL-15089 Bialystok, Poland

来源：

JOURNAL OF HEALTH SCIENCE | 2009年 / 55卷 / 02期

关键词：

poly(amidoamine) dendrimer; cytotoxicity; apoptosis; breast cancer cell; IN-VITRO CYTOTOXICITY; PAMAM DENDRIMERS; DRUG-DELIVERY; APOPTOSIS; MEMBRANE; DEATH; PHOSPHATIDYLSERINE; BIOCOMPATIBILITY; TRANSFECTION; TOXICITY;

D O I：

10.1248/jhs.55.169

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

In this study, we investigated the effect of amine- and hydroxyl-terminated Generation 2 and 3 (G2 and G3) poly(amidoamine) (PAMAM) dendrimers on the viability and induction of apoptosis in human MCF-7 and MDA-MB-231 breast cancer cells. It was shown that amino-terminated PAMAM dendrimers were cytotoxic, with IC50 value after 24 hr of incubation in MCF-7 and MDA-MB-231 breast cancer cells 153 +/- 3 mu M and 140 +/- 2 mu M for G2 PAMAM and 120 +/- 3 mu M and 99 +/- 2 mu M for G3 PAMAM, respectively. Experiments made with annexin V-fluorescein isothiocyanate (V-FITC) and detection of apoptosis by a fluorescent microscopy assay revealed that G2 and G3 PAMAM-NH2 dendrimers inhibited the proliferation of MCF-7 and MDA-MB-231 malignant cells by increasing the number of apoptotic and necrotic cells. The cytotoxic and apoptotic effect of G2 and G3 PAMAM-OH dendrimers was significantly weaker.

引用

页码：169 / 177

页数：9

共 34 条

[1] ANTI-NEOPLASTIC ACTIVITY OF POLY(L-LYSINE) WITH SOME ASCITES TUMOR-CELLS [J].