Loss of Halophytism by Interference with SOS1 Expression

被引:208
作者
Oh, Dong-Ha [1 ,2 ,3 ,4 ]
Leidi, Eduardo [5 ]
Zhang, Quan [1 ,2 ,6 ]
Hwang, Sung-Min [3 ,4 ]
Li, Youzhi [1 ,2 ,7 ]
Quintero, Francisco J. [5 ]
Jiang, Xingyu [5 ]
D'Urzo, Matilde Paino [8 ]
Lee, Sang Yeol [3 ,4 ]
Zhao, Yanxiu [6 ]
Bahk, Jeong Dong [3 ,4 ]
Bressan, Ray A. [8 ]
Yun, Dae-Jin [3 ,4 ]
Pardo, Jose M. [5 ]
Bohnert, Hans J. [1 ,2 ]
机构
[1] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA
[3] Gyeongsang Natl Univ, Div Appl Life Sci, Program BK21, Grad Sch, Jinju 660701, South Korea
[4] Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Grad Sch, Jinju 660701, South Korea
[5] CSIC, Inst Recursos Nat & Agrobiol, Seville 41012, Spain
[6] Shandong Normal Univ, Key Lab Plant Stress Res, Coll Life Sci, Jinan 250014, Peoples R China
[7] Guangxi Univ, Key Lab Minist Educ Microbial & Plant Genet Engn, Nanning 530005, Guangxi, Peoples R China
[8] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
基金
美国国家科学基金会;
关键词
THELLUNGIELLA-HALOPHILA; SALT TOLERANCE; ARABIDOPSIS-THALIANA; STRESS TOLERANCE; NA+ TRANSPORT; SODIUM ACCUMULATION; SIGNALING PATHWAY; SALINITY; GROWTH; ENDOCYTOSIS;
D O I
10.1104/pp.109.137802
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of the Thellungiella homolog ThSOS1 suppressed the salt-sensitive phenotype of a Saccharomyces cerevisiae strain lacking sodium ion (Na+) efflux transporters and increased salt tolerance of wild-type Arabidopsis. thsos1-RNAi lines of Thellungiella were highly salt sensitive. A representative line, thsos1-4, showed faster Na+ accumulation, more severe water loss in shoots under salt stress, and slower removal of Na+ from the root after removal of stress compared with the wild type. thsos1-4 showed drastically higher sodium-specific fluorescence visualized by CoroNa-Green, a sodium-specific fluorophore, than the wild type, inhibition of endocytosis in root tip cells, and cell death in the adjacent elongation zone. After prolonged stress, Na+ accumulated inside the pericycle in thsos1-4, while sodium was confined in vacuoles of epidermis and cortex cells in the wild type. RNAi-based interference of SOS1 caused cell death in the root elongation zone, accompanied by fragmentation of vacuoles, inhibition of endocytosis, and apoplastic sodium influx into the stele and hence the shoot. Reduction in SOS1 expression changed Thellungiella that normally can grow in seawater-strength sodium chloride solutions into a plant as sensitive to Na+ as Arabidopsis.
引用
收藏
页码:210 / 222
页数:13
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