Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguacu, State of Rio de Janeiro

被引：32

作者：

Lourenço-de-Oliveira, R

Honório, NA

Castro, MG

Schatzmayr, HG

Miagostovich, MP

Alves, JCR

Silva, WC

Leite, PJ

Nogueira, RMR

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Transmissores Hematozoarios, BR-21045900 Rio De Janeiro, RJ, Brazil

[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Flavivirus, BR-21045900 Rio De Janeiro, RJ, Brazil

[3] Fdn Nacl Saude, Nova Iguacu, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2002年 / 97卷 / 06期

关键词：

dengue; Aedes aegypti; Aedes albopictus; reverse transcription-polymerase chain reaction; virus isolation; Rio de Janeiro; Brazil;

D O I：

10.1590/S0074-02762002000600009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguacu, State of Rio de Janeiro, Brazil. Virus isolation in Ae albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of Ae. aegypti, despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that area. No viruses were detected in Ae. albopictus mosquitoes. This virological surveillance consists in a sentinel system alerting for dengue outbreaks.

引用

页码：799 / 800

页数：2

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