Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion

被引：378

作者：

Meissner, M

Schlüter, D

Soldati, D

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England

[2] Heidelberg Univ, Klinikum Mannheim, Inst Med Mikrobiol & Hyg, D-68167 Mannheim, Germany

来源：

SCIENCE | 2002年 / 298卷 / 5594期

关键词：

D O I：

10.1126/science.1074553

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.

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页码：837 / 840

页数：4

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