Rab35 Controls Actin Bundling by Recruiting Fascin as an Effector Protein

被引：135

作者：

Zhang, Jun ^{[1
,2
,3
,4
]}

Fonovic, Marko ^{[5
,6
,7
]}

Suyama, Kaye ^{[1
,2
,3
,4
]}

Bogyo, Matthew ^{[5
,6
]}

Scott, Matthew P. ^{[1
,2
,3
,4
]}

机构：

[1] Stanford Univ, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Biol Engn, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[5] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[6] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[7] Jozef Stefan Inst, Dept Biochem Mol & Struct Biol, Ljubljana 1000, Slovenia

来源：

SCIENCE | 2009年 / 325卷 / 5945期

关键词：

DROSOPHILA BRISTLES; SHORT FILAMENTS; CYTOKINESIS; EXPRESSION; SHAPE;

D O I：

10.1126/science.1174921

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Actin filaments are key components of the eukaryotic cytoskeleton that provide mechanical structure and generate forces during cell shape changes, growth, and migration. Actin filaments are dynamically assembled into higher-order structures at specified locations to regulate diverse functions. The Rab family of small guanosine triphosphatases is evolutionarily conserved and mediates intracellular vesicle trafficking. We found that Rab35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells. These effects were mediated by the actin-bundling protein fascin, which directly associated with active Rab35. Targeting Rab35 to the outer mitochondrial membrane triggered actin recruitment, demonstrating a role for an intracellular trafficking protein in localized actin assembly.

引用

页码：1250 / 1254

页数：5

共 25 条

[1] Cell-matrix adhesions differentially regulate fascin phosphorylation [J].