In situ hybridization studies of UDP-glucuronosyltransferase UGT1A6 expression in rat testis and brain

被引：17

作者：

Brands, A ^{[1
]}

Münzel, PA ^{[1
]}

Bock, KW ^{[1
]}

机构：

[1] Univ Tubingen, Inst Toxicol, D-72074 Tubingen, Germany

来源：

BIOCHEMICAL PHARMACOLOGY | 2000年 / 59卷 / 11期

关键词：

UDP-glucuronosyltransferase UGT1A6; in situ hybridization; testis; brain;

D O I：

10.1016/S0006-2952(00)00274-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

UDP-glucuronosyltransferases (UGTs), in addition to their role in overall pharmacokinetics, play important roles in local protection of cells against toxins and in the control of endogenous receptor ligands. UGT1A6, which conjugates planar phenols, appears to be expressed in many organs, but information on cell-specific expression in these organs is controversial or absent. Therefore, a non-isotopic in situ hybridization method was developed and applied to localize UGT1A6 expression in rat testis and brain. It was found that UGT1A6 is expressed in Sertoli cells and spermatogonia of rat testis and in brain neurons, in particular in hippocampal pyramidal cells and Purkinje cells of the cerebellum. BIOCHEM PHARMACOL 59;11:1441-1444, 2000. (C) 2000 Elsevier Science Inc.

引用

页码：1441 / 1444

页数：4

共 21 条

[1] Characterization of the UDP-glucuronosyltransferase isoenzyme expressed in rat ovary and its regulation by gonadotropins
Becedas, L
Lundgren, B
de Pierre, JW
[J]. BIOCHEMICAL JOURNAL, 1998, 332 : 51 - 55
[2] BOCK KW, 1992, MOL PHARMACOL, V42, P613
[3] PARACETAMOL GLUCURONIDATION BY RECOMBINANT RAT AND HUMAN PHENOL UDP-GLUCURONOSYLTRANSFERASES
BOCK, KW
FORSTER, A
GSCHAIDMEIER, H
BRUCK, M
MUNZEL, P
SCHARECK, W
FOURNELGIGLEUX, S
BURCHELL, B
[J]. BIOCHEMICAL PHARMACOLOGY, 1993, 45 (09) : 1809 - 1814
[4] INDUCTION OF PHASE-I AND PHASE-II DRUG-METABOLIZING ENZYME MESSENGER-RNA, PROTEIN, AND ACTIVITY BY BHA, ETHOXYQUIN, AND OLTIPRAZ
BUETLER, TM
GALLAGHER, EP
WANG, CH
STAHL, DL
HAYES, JD
EATON, DL
[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 1995, 135 (01) : 45 - 57
[5] 7-ethoxycoumarin deethylation activity in perfused isolated rat brain
Chikaoka, Y
Tamura, M
[J]. JOURNAL OF BIOCHEMISTRY, 1999, 125 (03) : 634 - 640
[6] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[7] Dutton GJ, 1980, GLUCURONIDATION DRUG
[8] Franklin TJ, 1996, CANCER RES, V56, P984
[9] A critical amino acid residue, Asp(446), in UDP-glucuronosyltransferase
Iwano, H
Yokota, H
Ohgiya, S
Yotumoto, N
Yuasa, A
[J]. BIOCHEMICAL JOURNAL, 1997, 325 : 587 - 591
[10] IYANAGI T, 1986, J BIOL CHEM, V261, P5607

← 1 2 3 →