Analysis of hydrolytic activity of a 65-kDa chitinase from the silkworm, Bombyx mori

被引：13

作者：

Abdel-Banat, BMA ^{[1
]}

Zhou, WM ^{[1
]}

Karasuda, S ^{[1
]}

Koga, D ^{[1
]}

机构：

[1] Yamaguchi Univ, Fac Agr, Dept Biol Sci, Biochem Lab, Yamaguchi 7538515, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 05期

关键词：

Bombyx mori; 65-kDa chitinase; N-acetylchitooligosaccharides; cleavage pattern; beta anomer formation;

D O I：

10.1271/bbb.66.1119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hydrolytic reactions of Bombyx mori 65-kDa chitinase with the short substrates, N-acetylchitooligosaccharides, were analyzed by HPLC. Analysis of the hydrolyzed products showed that the newly produced oligosaccharides are all beta anomers, suggesting that, similar to other family 18 glycosyl hydrolases, the 65-kDa chitinase acts in the retaining mechanism. Furthermore, the enzyme cleaves the N-acetylchitooligosaccharides mainly at the linkage between the second and the third GlcNAc moieties from the non-reducing end, while the other sites were cleaved in smaller proportions. Moreover, the initial reaction rates of the enzyme with the longer N-acetylchitooligosaccharides were higher than those with shorter ones. These results suggest that the enzyme is an endo-cleaving type and more efficient on the longer substrates.

引用

页码：1119 / 1122

页数：4

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