Acasp, a gene encoding a cathepsin D-like aspartic protease from the hookworm Ancylostoma caninum

被引:45
作者
Harrop, SA
Prociv, P
Brindley, PJ
机构
[1] QUEENSLAND INST MED RES, AUSTRALIAN CTR INT & TROP HLTH & NUTR, BANCROFT CTR, HERSTON, QLD 4029, AUSTRALIA
[2] UNIV QUEENSLAND, DEPT PARASITOL, ST LUCIA, QLD 4072, AUSTRALIA
基金
英国医学研究理事会;
关键词
D O I
10.1006/bbrc.1996.1503
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Intestinal infection with the zoonotic hookworm Ancylostoma caninum can provoke human eosinophilic enteritis. A cDNA was isolated from A. caninum, using an oligonucleotide primer designed to hybridize to the region encoding the consensus, catalytic site residues D(32)TGSSNLW of aspartic proteases. This novel cDNA encoded an aspartic protease zymogen of 422 amino acids, exhibiting 47% identity to the lysosomal aspartic protease of Aedes aegypti, 46% identity to the aspartic protease of Schistosoma japonicum and 48.5% to human cathepsin D. Its deduced structure differed from that of cathepsin D in the loop 2 ''flap,'' which holds the substrate at the active site, and by the presence of a COOH-terminal extension of similar to 30 residues. (C) 1996 Academic Press,Inc.
引用
收藏
页码:294 / 302
页数:9
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