Conditional expression of vaccinia virus genes in mammalian cell lines expressing the tetracycline repressor

被引：10

作者：

Hedengren-Olcott, M ^{[1
]}

Hruby, DE ^{[1
]}

机构：

[1] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2004年 / 120卷 / 01期

关键词：

vaccinia virus; conditional mutant; tetracycline controlled gene expression;

D O I：

10.1016/j.jviromet.2004.03.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new system was developed for producing conditional-lethal vaccinia virus mutants using the tetracycline controlled gene expression system from bacteria. The tetracycline resistance operon (tetO) sequence was placed between the promoter and coding sequence of the target gene of the virus. To regulate the expression of the target gene, the tetO-containing virus was used to infect a tetracycline repressor (TetR) expressing cell line. This method allowed isolation of a tetO-containing virus in the absence of the TetR thereby eliminating the risk of selecting for an inactivated viral tetR gene caused by recombination of the virus genome and improving the stability of the engineered mutants. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：9 / 12

页数：4

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