Cloning and characterization of rhesus monkey MCH-R1 and MCH-R2

被引：23

作者：

Fried, S ^{[1
]}

O'Neill, K ^{[1
]}

Hawes, BE ^{[1
]}

机构：

[1] Schering Plough Corp, Res Inst, CNSCV Biol Res, Kenilworth, NJ 07033 USA

来源：

PEPTIDES | 2002年 / 23卷 / 08期

关键词：

MCH; receptor; G protein; rhesus monkey; intracellular signaling; amino acid sequence; nucleic acid sequence;

D O I：

10.1016/S0196-9781(02)00077-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhesus monkey MCH-R1 and MCH-R2 receptors were cloned. Amino acid homology is 98.8% between monkey and human MCH-R1, while monkey and human MCH-R2 are 98% homologous. Binding and intracellular signaling characteristics of the monkey receptors were compared with the human homologues. The results demonstrate that MCH binds to the monkey MCH-R1 receptor with a K-d of 6.5 nM and monkey MCH-R2 with a K-d of 2.2 nM similar to K-d values for human MCH-R1 and MCH-R2. Additionally, monkey MCH-R1 couples through G(i)/G(o) and G(q)-type G proteins similar to human MCH-R1 whereas monkey and human MCH-R2 utilize the G(q) signaling pathway. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：1401 / 1408

页数：8

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