Iron-related transcriptomic variations in CaCo-2 cells, an in vitro model of intestinal absorptive cells

被引:13
作者
Chicault, Celine
Toutain, Bertrand
Monnier, Annabelle
Aubry, Marc
Fergelot, Patricia
Le Treut, Andre
Galibert, Marie-Dominique
Mosser, Jean
机构
[1] Univ Rennes 1, CNRS, UMR 6061 Genet & Dev, F-35043 Rennes, France
[2] GFAS, IFR140, Grp Oncogenom, Fac Med, Rennes, France
[3] IFR 140, OUEST Genopole, Rennes, France
关键词
enterocytes; microarray analysis of gene expression; hemin overload; iron deficiency;
D O I
10.1152/physiolgenomics.00297.2005
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Regulation of iron absorption by duodenal enterocytes is essential for the maintenance of homeostasis by preventing iron deficiency or overload. Despite the identification of a number of genes implicated in iron absorption and its regulation, it is likely that further factors remain to be identified. For that purpose, we used a global transcriptomic approach, using the CaCo-2 cell line as an in vitro model of intestinal absorptive cells. Pangenomic screening for variations in gene expression correlating with intracellular iron content allowed us to identify 171 genes. One hundred nine of these genes are clustered into five types of expression profile. This is the first time that most of these genes have been associated with iron metabolism. Functional annotation of these five clusters indicates potential links between the immune response, proteolysis processes, and iron depletion. In contrast, iron overload is associated with cellular metabolism, especially that of lipids and glutathione involving redox function and electron transfer.
引用
收藏
页码:55 / 67
页数:13
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