Direct uptake of nitrogen by Pfiesteria piscicida and Pfiesteria shumwayae, and nitrogen nutritional preferences

被引:32
作者
Glibert, Patricia M.
Burkholder, JoAnn M.
Parrow, Matthew W.
Lewitus, Alan J.
Gustafson, Daniel E.
机构
[1] Univ Maryland, Horn Point Environm Lab, Ctr Environm Sci, Cambridge, MD 21613 USA
[2] N Carolina State Univ, Ctr Appl Aquat Ecol, Raleigh, NC 27695 USA
[3] Univ S Carolina, Belle W Baruch Inst Marine Sci & Coastal Res, Baruch Marine Lab, Georgetown, SC 29442 USA
[4] Marine Resources Res Inst, S Carolina Dept Nat Resources, Hollings Marine Lab, Charleston, SC 29412 USA
基金
美国国家航空航天局;
关键词
Pfiesteria; nitrogen nutrition; heterotrophy; toxigenic dinoflagellates;
D O I
10.1016/j.hal.2006.04.009
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
The rates of uptake of a range of forms of nitrogenous nutrients were measured in cultures of Pfiesteria piscicida and Pfiesteria shumwayae maintained. at varying physiological states. The measured rates of dissolved N uptake under some conditions approached the rates of N uptake that are achieved through phagotrophy. Rates of dissolved N uptake by P. piscicida contributed < 10% of the cellular N of flagellated cells feeding on algae, but were equal to or greater than phagotrophic N acquisition in cells recently removed from fish cultures. Specific N uptake rates (V, h(-1)) were higher for cells that were maintained on algal prey for long periods (months) than those that were grown with live fish. However, rates of N uptake on a cellular basis for cells grown on or recently removed from fish were comparable to those maintained on algal prey, likely reflecting differences in the sizes of cells of different physiological condition. Preferences for form of N generally followed a decreasing trend of amino acids > urea > NH4+ > NO3-. Nitrate consistently was not a preferred form of N. Although Pfiesteria spp. are often found in eutrophic environments, the relationship between Pfiesteria spp. and nutrient availability is likely to be primarily indirect, mediated through the production of various prey on which Pfiesteria spp. feed. These findings also confirm, however, that when dissolved N concentrations are elevated, they can contribute to the supplemental nutrition of these cells, and thus may provide a significant source of N to Pfiesteria spp. in nature. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:380 / 394
页数:15
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