Separating nucleation and growth in protein crystallization using dynamic light scattering

被引：34

作者：

Saridakis, E

Dierks, K

Moreno, A

Dieckmann, MWM

Chayen, NE ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Biomed Sci, Biol Struct & Funct Sect, London SW7 2AZ, England

[2] Dierks & Partner Syst Tech, D-20257 Hamburg, Germany

[3] Univ Nacl Autonoma Mexico, Inst Quim, Dept Bioquim, Mexico City, DF, Mexico

[4] European Space Agcy, ESTEC, NL-2200 AG Noordwijk, Netherlands

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

protein crystallization; nucleation; microbatch; dynamic light scattering; biological macromolecules; crystallogenesis;

D O I：

10.1107/S0907444902014348

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.

引用

页码：1597 / 1600

页数：4

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