Simultaneous production of high activities of thermostable endoglucanase and β-glucosidase by the wild thermophilic fungus Thermoascus aurantiacus

被引:69
作者
Gomes, I
Gomes, J
Gomes, DJ
Steiner, W
机构
[1] Graz Univ Technol, Inst Biotechnol, A-8010 Graz, Austria
[2] Bangladesh Jute Res Inst, Dhaka, Bangladesh
[3] Univ Dhaka, Dept Microbiol, Dhaka, Bangladesh
关键词
D O I
10.1007/s002530051642
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The culture-medium composition was optimised, on a shake-flask scale, for simultaneous production of high activities of endoglucanase and beta-glucosidase by Thermoascus aurantiacus using statistical factorial designs. The optimised medium containing 40.2 g l(-1) Solka Flee as the carbon source and 9 g l(-1) soymeal as the organic nitrogen source yielded 1130 nkat ml(-1) endoglucanase and 116 nkat ml(-1) beta-glucosidase activities after 264 h as shake cultures. In addition, good levels of beta-xylanase (3479 nkat ml(-1)) and low levels of filter-paper cellulase, beta-xylosidase, alpha-L-arabinofuranosidase, beta-mannanase, beta-mannosidase, alpha-galactosidase and beta-galactosidase were detected. Batch fermentation in a 5-1 laboratory fermenter using the optimised medium allowed the production of 940 nkat ml(-1) endoglucanase and 102 nkat ml(-1) beta-glucosidase in 192 h. Endoglucanase and beta-glucosidase showed optimum activity at pH 4.5 and pH 5, respectively, and they displayed optimum activity at 75 degrees C. Endoglucanase and beta-glucosidase showed good stability at pH values 4-8 and 4-7, respectively, after a prolonged incubation (48 h at 50 degrees C). Endoglucanase had half-lives of 98 h at 70 degrees C and 4.1 h at 75 degrees C, while beta-glucosidase had half-lives of 23.5 h at 70 degrees C and 1.7 h at 75 degrees C. Alkali-treated bagasse, steam-treated wheat straw, Solka flee and Sigmacell 50 were 66, 48.5, 33.5 and 14.4% hydrolysed by a crude enzyme complex of T. aurantiacus in 50 h.
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页码:461 / 468
页数:8
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