SNX5, a new member of the sorting nexin family, binds to the Fanconi anemia complementation group A protein

被引:53
作者
Otsuki, T
Kajigaya, S
Ozawa, E
Liu, JM
机构
[1] Jichi Med Sch, Dept Hematol, Minami Kawachi, Tochigi 3290498, Japan
[2] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA
关键词
Fanconi anemia; yeast two-hybrid; sorting nexin; immunoprecipitation;
D O I
10.1006/bbrc.1999.1731
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The function of the Fanconi anemia complementation group A (FANCA) protein remains unclear. To investigate possible protein-protein interactions, we performed yeast two-hybrid screening using a FANCA fragment as bait. Sorting nexin 5 (SNX5), a new member of the human SNX family, was identified as a putative FANCA-binding protein. The interaction between FANCA and SNX5 was confirmed by immunoprecipitation studies. All members of the SNX family have a characteristic amino acid region termed the phox homology (PX) domain. Deletion mutant analysis indicated that the PX domain is not required for binding to FANCA. The SNX proteins are thought to play an important role in receptor trafficking between organelles. We found that overexpression of SNX5 increased FANCA protein levels. Northern blot analysis of SNX5 showed the presence of alternatively spliced transcripts and different expression patterns in various human cancer cell lines and normal tissues. Further studies are needed to elucidate the functional significance of FANCA and SNX5 binding; however, we speculate that FANCA may affect SNX5 traffic with cell surface receptors. (C) 1999 Academic Press.
引用
收藏
页码:630 / 635
页数:6
相关论文
共 24 条
  • [1] Positional cloning of the Fanconi anaemia group A gene
    Apostolou, S
    Whitmore, SA
    Crawford, J
    Lennon, G
    Sutherland, GR
    Callen, DF
    Ianzano, L
    Savino, M
    DApolito, M
    Notarangelo, A
    Memeo, E
    Piemontese, MR
    Zelante, L
    Savoia, A
    Gibson, RA
    Tipping, AJ
    Morgan, NV
    Hassock, S
    Jansen, S
    deRavel, TJ
    VanBerkel, C
    Pronk, JC
    Easton, DF
    Mathew, CG
    Levran, O
    Verlander, PC
    Batish, SD
    Erlich, T
    Auerbach, AD
    CletonJansen, AM
    Moerland, EW
    Cornelisse, CJ
    Doggett, NA
    Deaven, LL
    Moyzis, RK
    [J]. NATURE GENETICS, 1996, 14 (03) : 324 - 328
  • [2] The Fanconi anaemia group G gene FANCG is identical with XRCC9
    de Winter, JP
    Waisfisz, Q
    Rooimans, MA
    van Berkel, CGM
    Bosnoyan-Collins, L
    Alon, N
    Carreau, M
    Bender, O
    Demuth, I
    Schindler, D
    Pronk, JC
    Arwert, F
    Hoehn, H
    Digweed, M
    Buchwald, M
    Joenje, H
    [J]. NATURE GENETICS, 1998, 20 (03) : 281 - 283
  • [3] EKENA K, 1995, MOL CELL BIOL, V15, P1671
  • [4] Garcia-Higuera I, 1999, MOL CELL BIOL, V19, P4866
  • [5] Identification of a family of sorting nexin molecules and characterization of their association with receptors
    Haft, CR
    Sierra, MD
    Barr, VA
    Haft, DH
    Taylor, SI
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (12) : 7278 - 7287
  • [6] A sorting nexin-1 homologue, vps5p, forms a complex with vps17p and is required for recycling the vacuolar protein-sorting receptor
    Horazdovsky, BF
    Davies, BA
    Seaman, MNJ
    McLaughlin, SA
    Yoon, S
    Emr, SD
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (08) : 1529 - 1541
  • [7] ISHIDA R, 1982, CANCER RES, V42, P4000
  • [8] CLASSIFICATION OF FANCONI-ANEMIA PATIENTS BY COMPLEMENTATION ANALYSIS - EVIDENCE FOR A 5TH GENETIC SUBTYPE
    JOENJE, H
    LO TEN FOE, JR
    OOSTRA, AB
    VANBERKEL, CGN
    ROOIMANS, MA
    SCHROEDERKURTH, T
    WEGNER, RD
    GILLE, JJP
    BUCHWALD, M
    ARWERT, F
    [J]. BLOOD, 1995, 86 (06) : 2156 - 2160
  • [9] Evidence for at least eight Fanconi anemia genes
    Joenje, H
    Oostra, AB
    Wijker, M
    diSumma, FM
    vanBerkel, CGM
    Rooimans, MA
    Ebell, W
    vanWeel, M
    Pronk, JC
    Buchwald, M
    Arwert, F
    [J]. AMERICAN JOURNAL OF HUMAN GENETICS, 1997, 61 (04) : 940 - 944
  • [10] KAISER TN, 1982, CYTOMETRY, V2, P291