Mycobacterium kyorinense sp nov., a novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens

被引：25

作者：

Okazaki, Mitsuhiro ^{[1
]}

Ohkusu, Kiyofumi ^{[2
]}

Hata, Hiroyuki ^{[2
]}

Ohnishi, Hiroaki ^{[1
]}

Sugahara, Keiko ^{[3
]}

Kawamura, Chizuko ^{[4
]}

Fujiwara, Nagatoshi ^{[5
]}

Matsumoto, Sohkichi ^{[5
]}

Nishiuchi, Yukiko ^{[6
]}

Toyoda, Kouichi ^{[7
]}

Saito, Hajime ^{[8
]}

Yonetani, Shota ^{[1
]}

Fukugawa, Yoko ^{[1
]}

Yamamoto, Masayuki ^{[9
]}

Wada, Hiroo ^{[9
]}

Sejimo, Akiko ^{[3
]}

Ebina, Akio ^{[4
]}

Goto, Hajime ^{[9
]}

Ezaki, Takayuki ^{[2
]}

Watanabe, Takashi ^{[1
]}

机构：

[1] Kyorin Univ, Sch Med, Dept Lab Med, Mitaka, Tokyo 1818611, Japan

[2] Gifu Univ, Grad Sch Med, Dept Microbiol Regenerat & Adv Med Sci, Gifu 5011194, Japan

[3] Tokyo Natl Hosp, Natl Hosp Org, Kiyose, Tokyo 2040023, Japan

[4] Aomori Prefectural Hosp, Cent Lab, Aomori 0308553, Japan

[5] Osaka City Univ, Grad Sch Med, Dept Host Def, Abeno Ku, Osaka 5458585, Japan

[6] Osaka City Univ, Sch Med, Toneyama Inst TB Res, Osaka 545, Japan

[7] Kyokuto Pharmaceut Ind Co Ltd, Chuuou Ku, Tokyo 1030024, Japan

[8] Hiroshima Environm & Hlth Assoc, Hlth Sci Ctr, Naka Ku, Hiroshima 7308631, Japan

[9] Kyorin Univ, Sch Med, Dept Resp, Mitaka, Tokyo 1818611, Japan

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2009年 / 59卷

关键词：

MICRODILUTION WELLS; IDENTIFICATION; HYBRIDIZATION; POLYMERASE;

D O I：

10.1099/ijs.0.000760-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel, non-pigmented, slow-growing mycobacterium was identified on the basis of biochemical and nucleic acid analyses, as well as growth characteristics. Three isolates were cultured from clinical samples (two from sputum and one from pus in lymph nodes) obtained from three immunocompetent patients with infections. Bacterial growth occurred at 28-42 degrees C on Middlebrook 7H11-OADC agar. The isolates showed negative results for Tween hydrolysis, nitrate reductase, semiquantitative catalase, urease activity, 3 day arylsulfatase activity, pyrazinamidase, tellurite reduction and niacin accumulation tests, but positive results for 14 day arylsulfatase activity and heat-stable catalase tests. The isolates contained alpha-, keto-, and dicarboxymycolates in their cell walls. Sequence analysis revealed that all isolates had identical, unique 16S rRNA sequences. Phylogenetic analysis of the 16S rRNA, rpoB, hsp65 and sodA gene sequences confirmed that these isolates are unique but closely related to Mycobacterium celatum. DNA-DNA hybridization of the isolates demonstrated less than 50% reassociation with M. celatum and Mycobacterium branderi. On the basis of these findings, a novel species designated Mycobacterium kyorinense sp. nov. is proposed. The type strain is KUM 060204(T) (=JCM 15038(T)=DSM 45166(T)).

引用

页码：1336 / 1341

页数：6

共 18 条

[1]

*AM LUNG ASS MED S, 1997, AM J RESP CRIT CARE, V156, pS1

[2]

[Anonymous], 2003, Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes

[3]

Approved Standard. NCCLS document M24-A. NCCLS, 940 West Valley Road

[4] GENETIC AND TRANSCRIPTIONAL ORGANIZATION OF THE REGION ENCODING THE BETA-SUBUNIT OF BACILLUS-SUBTILIS RNA-POLYMERASE [J].

BOOR, KJ ;

DUNCAN, ML ;

PRICE, CW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (35) :20329-20336

[5] CHARACTERIZATION OF A DISTINCT GROUP OF SLOWLY GROWING MYCOBACTERIA BY BIOCHEMICAL TESTS AND LIPID ANALYSES [J].

BRANDER, E ;

JANTZEN, E ;

HUTTUNEN, R ;

JULKUNEN, A ;

KATILA, ML .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :1972-1975

[6]

BROWNELLIOTT BA, 2005, MANDELL DOUGLAS BENN, V2, P2909

[7] MYCOBACTERIUM-CELATUM SP-NOV [J].

BUTLER, WR ;

OCONNOR, SP ;

YAKRUS, MA ;

SMITHWICK, RW ;

PLIKAYTIS, BB ;

MOSS, CW ;

FLOYD, MM ;

WOODLEY, CL ;

KILBURN, JO ;

VADNEY, FS ;

GROSS, WM .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (03) :539-548

[8]

Chapin KC, 2007, MANUAL OF CLINICAL MICROBIOLOGY, 9TH ED, P182

[9] Mycobacterium mageritense sp nov [J].

Domenech, P ;

Jimenez, MS ;

Menendez, MC ;

Bull, TJ ;

Samper, S ;

Manrique, A ;

Garcia, MJ .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (02) :535-540

[10] SIMPLE GENETIC METHOD TO IDENTIFY VIRIDANS GROUP STREPTOCOCCI BY COLORIMETRIC DOT HYBRIDIZATION AND FLUOROMETRIC HYBRIDIZATION IN MICRODILUTION WELLS [J].

EZAKI, T ;

HASHIMOTO, Y ;

TAKEUCHI, N ;

YAMAMOTO, H ;

LIU, SL ;

MIURA, H ;

MATSUI, K ;

YABUUCHI, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (09) :1708-1713

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