The fluorescence detection of superoxide radical using hydroethidine could be complicated by the presence of heme proteins

被引：42

作者：

Papapostolou, L ^{[1
]}

Patsoukis, N ^{[1
]}

Georgiou, CD ^{[1
]}

机构：

[1] Univ Patras, Dept Biol, Patras 26500, Greece

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 332卷 / 02期

关键词：

Cytochromes; hemoglobin; hydroethidine; ethidium; mitochondria; myoglobin; free radicals;

D O I：

10.1016/j.ab.2004.06.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study shows that hydroethidine (HE) used for the qualitative detection of superoxide anion can also be oxidized by heme proteins such as the mitochondrial cytochromes, hemoglobin, and myoglobin, forming spectrally nonhomogenous mixtures of HE-derived products of various oxidation states. All oxidation products show excitation/emission peaks (490-495/580-600nm) near the excitation/emission peaks (475/570nm) of the HE-superoxide oxidation product, and this may pose serious interference problems to the fluorescent detection of the superoxide radical. This paper discusses possible precautionary steps that should be taken to minimize the interfering problems in the HE-superoxide assay and suggests its use mainly for reactive oxygen species detection. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：290 / 298

页数：9

共 14 条

[1] DAMAGE TO MITOCHONDRIAL ELECTRON-TRANSPORT AND ENERGY COUPLING BY VISIBLE-LIGHT [J].