Glucuronic acid directly linked to galacturonic acid in the rhamnogalacturonan backbone of beet pectins

被引：60

作者：

Renard, CMGC ^{[1
]}

Crépeau, MJ ^{[1
]}

Thibault, JF ^{[1
]}

机构：

[1] INRA, Ctr Rech Agroalimentaire Nantes, Unite Rech Polysaccharides Leurs Org & Interact, F-44026 Nantes, France

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 266卷 / 02期

关键词：

pectin; rhamnogalacturonan; glucuronic acid; sugar beet;

D O I：

10.1046/j.1432-1327.1999.00896.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sugar-beet pulp was de-esterified and submitted to 72 h hydrolysis by 0.1 M HCl at 80 degrees C. Oligomers containing a single glucuronic acid (G1cA) moiety in addition to n(greater than or equal to 2) repeats of the dimer -->4)-alpha-D-Ga1pA-(1-->2)-alpha-L-Rhap-(1--> were isolated from the hydrolysate by ion-exchange and gel-permeation. Glycosyl linkage composition analysis and H-1 NMR studies indicated that the GlcA was attached to O-3 of a galacturonic acid (Ga1A) residue, as shown for the two pentamers beta-D-G1cpA-(1-->3)-alpha-D-Ga1pA-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-Ga1pA-(1-->2)-L-Rhap and alpha-D-Ga1pA-(1-->2)-alpha-L-Rhap-(1-->4)-[beta-D-G1cpA-(1-->3)]-alpha-D-Ga1pA-(1-->2)-L-Rhap. Substitution by G1cA was estimated as occurring on one Ga1A residue out of 72 in the rhamnogalacturonan fraction of the backbone of beet pectins.

引用

页码：566 / 574

页数：9

共 39 条

[1]

AN JA, 1994, CARBOHYD RES, V252, P235

[2] PECTIC SUBSTANCES FROM LUCERNE (MEDICAGO SATIVA) .2. ACIDIC OLIGOSACCHARIDES FROM PARTIAL HYDROLYSIS OF LEAF AND STEM PECTIC ACIDS [J].