Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substrates for nerve tissue engineering

被引:260
作者
Prabhakaran, Molamma P. [1 ]
Venugopal, Jayarama Reddy [1 ]
Ramakrishna, Seeram [1 ]
机构
[1] Natl Univ Singapore, Div Bioengn, Nanosci & Nanotechnol Initiat, Singapore 117576, Singapore
基金
英国医学研究理事会;
关键词
Mesenchymal stem cells; Neuronal cells; Nerve tissue engineering; Electrospun nanofibers; Inducing factors; Differentiation; MARROW STROMAL CELLS; BONE-MARROW; NEURAL CELLS; OSTEOGENIC DIFFERENTIATION; BIOMIMETIC MATERIALS; INDUCTION; SCAFFOLDS; LINEAGES; GROWTH;
D O I
10.1016/j.biomaterials.2009.05.057
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Bone marrow Mesenchymal stem cells capable of differentiating into neuronal cells on engineered nanofibrous scaffolds have great potential for bionanomaterial-cell transplantation therapy of neuro-degenerative diseases and injuries of the nervous system. MSCs have been the highlight of many tissue engineering studies mainly because of their multipotential properties. We investigated the potential of human bone marrow derived Mesenchymal stem cells (MSCs) for neuronal differentiation in vitro on poly(L-lactic acid)-co-poly-(3-caprolactone)/Collagen (PLCL/Coll) nanofibrous scaffolds. PLCL and PLCL/Coll nanofibrous scaffolds were fabricated by electrospinning process and their chemical and mechanical characterizations were carried out using SEM, contact angle, FIR, and tensile instrument. The differentiation of MSCs was carried out using neuronal inducing factors including beta-mercaptoethanol, epidermal growth factor, nerve growth factor and brain derived growth factor in DMEM/F12 media. The proliferations of MSCs evaluated by MTS assay showed that the cells grown on PLCL/Coll nanofibrous scaffolds were comparatively higher (80%) than those on PLCL Scanning electron microscopy results showed that MSCs differentiated on PLCL/Coll nanofibrous scaffolds showed neuronal morphology, with multipolar elongations and expressed neurofilament and nestin protein by immuno-fluorescent microscopy. Our studies on the differentiation of MSCs to neuronal cells on nanofibrous scaffolds suggest their potential application towards nerve regeneration. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4996 / 5003
页数:8
相关论文
共 38 条
[1]   Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice [J].
Barberi, T ;
Klivenyi, P ;
Calingasan, NY ;
Lee, H ;
Kawamata, H ;
Loonam, K ;
Perrier, AL ;
Bruses, J ;
Rubio, ME ;
Topf, N ;
Tabar, V ;
Harrison, NL ;
Beal, MF ;
Moore, MAS ;
Studer, L .
NATURE BIOTECHNOLOGY, 2003, 21 (10) :1200-1207
[2]   Mechanical properties of acellular peripheral nerve [J].
Borschel, GH ;
Kia, KF ;
Kuzon, WM ;
Dennis, RG .
JOURNAL OF SURGICAL RESEARCH, 2003, 114 (02) :133-139
[3]   Coaxing bone marrow stromal mesenchymal stem cells towards neuronal differentiation: progress and uncertainties [J].
Chen, Y. ;
Teng, F. Y. H. ;
Tang, B. L. .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2006, 63 (14) :1649-1657
[4]   Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro [J].
Choong, P-F ;
Mok, P-L ;
Cheong, S-K ;
Leong, C-F ;
Then, K-Y .
CYTOTHERAPY, 2007, 9 (02) :170-183
[5]   In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP [J].
Deng, WW ;
Obrocka, M ;
Fischer, I ;
Prockop, DJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 282 (01) :148-152
[6]   Electrospun poly(ε-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering [J].
Ghasemi-Mobarakeh, Laleh ;
Prabhakaran, Molamma P. ;
Morshed, Mohammad ;
Nasr-Esfahani, Mohammad-Hossein ;
Ramakrishna, Seeram .
BIOMATERIALS, 2008, 29 (34) :4532-4539
[7]  
Grinnell F, 1978, Int Rev Cytol, V53, P65
[8]   Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells [J].
Hermann, A ;
Gastl, R ;
Liebau, S ;
Popa, MO ;
Fiedler, J ;
Boehm, BO ;
Maisel, M ;
Lerche, H ;
Schwarz, J ;
Brenner, R ;
Storch, A .
JOURNAL OF CELL SCIENCE, 2004, 117 (19) :4411-4422
[9]   Proliferation and differentiation of mesenchymal stem cells using self-assembled peptide amphiphile nanofibers [J].
Hosseinkhani, Hossein ;
Hosseinkhani, Mohsen ;
Kobayashi, Hisatoshi .
BIOMEDICAL MATERIALS, 2006, 1 (01) :8-15
[10]   Osteogenic differentiation of mesenchymal stem cells in self-assembled peptide-amphiphile nanofibers [J].
Hosseinkhani, Hossein ;
Hosseinkhani, Mohsen ;
Tian, Furong ;
Kobayashi, Hisatoshi ;
Tabata, Yasuhiko .
BIOMATERIALS, 2006, 27 (22) :4079-4086