Quantitative analysis of genetically modified organisms (GMO) in processed food by PCR-based methods

被引：82

作者：

Wurz, A ^{[1
]}

Bluth, A ^{[1
]}

Zeltz, P ^{[1
]}

Pfeifer, C ^{[1
]}

Willmund, R ^{[1
]}

机构：

[1] Gene Scan GmbH, D-79111 Freiburg, Germany

来源：

FOOD CONTROL | 1999年 / 10卷 / 06期

关键词：

genetically modified organism; food; PCR; quantitative analysis;

D O I：

10.1016/S0956-7135(99)00080-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Two different PCR-based approaches for the quantitative analysis of genetically modified organism (GMO) - components in foods are presented using Soybean derived samples as an example. The first method - a double competitive PCR - is well suited to determine threshold levels of GMO content in food,The other - PCR on-line measurement - is suited to determine ratios of transgenic versus non-transgenic component. Both methods provide a means to alleviate the problems of standardisation encountered with simple qualitative PCR approaches and will allow to cope with threshold levels for GMO, once issued by legislative bodies. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：385 / 389

页数：5