Isostaticity and controlled force transmission in the cytoskeleton: A model awaiting experimental evidence

被引:23
作者
Blumenfeld, Raphael
机构
[1] Cavendish Lab, Cambridge CB3 0HE, England
[2] Univ London Imperial Coll Sci & Technol, London, England
关键词
LIVING ENDOTHELIAL-CELLS; MECHANICS; TENSEGRITY; PRESTRESS; ARCHITECTURE; PROPAGATION; FIBROBLASTS; FILAMENTS; SHAPE;
D O I
10.1529/biophysj.105.076703
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A new model is proposed for force transmission through the cytoskeleton (CSK). A general discussion is first presented on the physical principles that underlie the modeling of this phenomenon. Some fundamental problems of conventional models-continuous and discrete-are examined. It is argued that mediation of focused forces is essential for good control over intracellular mechanical signals. The difficulties of conventional continuous models in describing such mediation are traced to a fundamental assumption rather than to their being continuous. Relevant advantages and disadvantages of continuous and discrete modeling are discussed. It is concluded that favoring discrete models is based on two misconceptions, which are clarified. The model proposed here is based on the idea that focused propagation of mechanical stimuli in frameworks over large distances (compared to the mesh size) can only occur when considerable regions of the CSK are isostatic. The concept of isostaticity is explained and a recently developed continuous isostaticity theory is briefly reviewed. The model enjoys several advantages: it leads to good control over force mediation; it explains nonuniform stresses and action at a distance; it is continuous, making it possible to model force propagation over long distances; and it enables prediction of individual force paths. To be isostatic, or nearly so, CSK networks must possess specific structural characteristics, and these are quantified. Finally, several experimental observations are interpreted using the new model and implications are discussed. It is also suggested that this approach may give insight into the dynamics of reorganization of the CSK. Many of the results are amenable to experimental measurements, providing a testing ground for the proposed picture, and generic experiments are suggested.
引用
收藏
页码:1970 / 1983
页数:14
相关论文
共 38 条
[1]   MECHANISMS OF CELL-SHAPE CHANGE - THE CYTOMECHANICS OF CELLULAR-RESPONSE TO CHEMICAL ENVIRONMENT AND MECHANICAL LOADING [J].
ADAMS, DS .
JOURNAL OF CELL BIOLOGY, 1992, 117 (01) :83-93
[2]  
Ball R. C., 1999, STRUCTURES DYNAMICS
[3]   Stress field in granular systems: Loop forces and potential formulation [J].
Ball, RC ;
Blumenfeld, R .
PHYSICAL REVIEW LETTERS, 2002, 88 (11) :4-115505
[4]   Local measurements of viscoelastic parameters of adherent cell surfaces by magnetic bead microrheometry [J].
Bausch, AR ;
Ziemann, F ;
Boulbitch, AA ;
Jacobson, K ;
Sackmann, E .
BIOPHYSICAL JOURNAL, 1998, 75 (04) :2038-2049
[5]   Stresses in isostatic granular systems and emergence of force chains [J].
Blumenfeld, R .
PHYSICAL REVIEW LETTERS, 2004, 93 (10) :108301-1
[6]   Stress transmission in planar disordered solid foams [J].
Blumenfeld, R .
JOURNAL OF PHYSICS A-MATHEMATICAL AND GENERAL, 2003, 36 (10) :2399-2411
[7]   Cellular control lies in the balance of forces [J].
Chicurel, ME ;
Chen, CS ;
Ingber, DE .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (02) :232-239
[8]   A tensegrity structure with buckling compression elements: Application to cell mechanics [J].
Coughlin, MF ;
Stamenovic, D .
JOURNAL OF APPLIED MECHANICS-TRANSACTIONS OF THE ASME, 1997, 64 (03) :480-486
[9]   Simulations of the erythrocyte cytoskeleton at large deformation. II. Micropipette aspiration [J].
Discher, DE ;
Boal, DH ;
Boey, SK .
BIOPHYSICAL JOURNAL, 1998, 75 (03) :1584-1597
[10]  
DONG C, 1991, BIORHEOLOGY, V28, P557