Preparation of nanocones for immobilizing DNA probe by a low-temperature plasma plume

Using allylamine monomer, a matrix of nanocones was fabricated by applying a low-temperature plasma plume without any catalysts and template. This nanocone acted as an adhesion layer immobilizing DNA probe for DNA hybridization assay. A simple conceptual model to describe the growth of the nanocones was also developed. The highest density of amino-labeled DNA probe was about 1.6 pM/cm(2) confirmed by the dyed oligonucleotide, and each nanocone contained nearly 3x10(2) amine groups. This strategy provides a robust procedure to immobilize DNA, which is a very useful substrate for fabricating nanobiosensors. (c) 2006 American Institute of Physics.