Crystallization of biological macromolecules using agarose gel

被引：51

作者：

Biertümpfel, C ^{[1
]}

Basquin, J ^{[1
]}

Suck, D ^{[1
]}

Sauter, C ^{[1
]}

机构：

[1] European Mol Biol Lab, Struct Computat Biol Programme, D-69012 Heidelberg, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

agarose gel; crystallization; biological macromolecule; counter-diffusion;

D O I：

10.1107/S0907444902012738

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gellified media prevent convection and crystal sedimentation, and provide an attractive growth environment for optimising biological crystals. Agarose gels are particularly easy to use and they are compatible with most of the common crystallization methods. They also offer new possibilities like counter-diffusion techniques. This paper gives a brief overview of their general properties and presents an application of a counter-diffusion setup combining agarose gel and capillaries to the crystallization of proteins and protein/nucleic acid complexes.

引用

页码：1657 / 1659

页数：3

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