Time-resolved responses to chemoattractant, characteristic of the front and tall of Dictyostelium cells

被引:27
作者
Etzrodt, Martin
Ishikawa, Hellen C. F.
Dalous, Jeremie
Mueller-Taubenberger, Annette
Bretschneider, Till
Gerisch, Guenther
机构
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
[2] CEA Grenoble, Dept Reponse & Dynam Cellulaires, F-38054 Grenoble 09, France
来源
FEBS LETTERS | 2006年 / 580卷 / 28-29期
关键词
Aip1; Arp2/3; coronin; chemotaxis; myosin; Dictyostelium;
D O I
10.1016/j.febslet.2006.11.031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In a gradient of chemoattractant, Dictyostelium cells are orientated with their front directed toward the source and their tail pointing into the opposite direction. The front region is specified by the polymerization of actin and the tail by the recruitment of filamentous myosin-II. We have dissected these front and tail responses by exposing cells to an upshift of cyclic AMP. A sharp rise and fall of polymerized actin within 10 s is accompanied by the recruitment of proteins involved in turning actin polymerization on or off. The cortical accumulation of myosin-II starts when the front response has declined, supporting the concept of divergent signal transmission and adaptation pathways. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:6707 / 6713
页数:7
相关论文
共 45 条
[1]   Signaling to cytoskeletal dynamics during chemotaxis [J].
Affolter, M ;
Weijer, CJ .
DEVELOPMENTAL CELL, 2005, 9 (01) :19-34
[2]   The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension [J].
Bailly, M ;
Ichetovkin, I ;
Grant, W ;
Zebda, N ;
Machesky, LM ;
Segall, JE ;
Condeelis, J .
CURRENT BIOLOGY, 2001, 11 (08) :620-625
[3]   CHEMOATTRACTANT-ELICITED INCREASES IN MYOSIN PHOSPHORYLATION IN DICTYOSTELIUM [J].
BERLOT, CH ;
SPUDICH, JA ;
DEVREOTES, PN .
CELL, 1985, 43 (01) :307-314
[4]   Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cells [J].
Bretschneider, T ;
Diez, S ;
Anderson, K ;
Heuser, J ;
Clarke, M ;
Müller-Taubenberger, A ;
Köhler, J ;
Gerisch, G .
CURRENT BIOLOGY, 2004, 14 (01) :1-10
[5]   Dictyostelium discoideum adhesion and motility under shear flow:: experimental and theoretical approaches [J].
Bruckert, F ;
Décavé, E ;
Garrivier, D ;
Cosson, P ;
Bréchet, Y ;
Fourcade, B ;
Satre, M .
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 2002, 23 (7-8) :651-658
[6]  
CHAI L, 2005, J BIOL CHEM, V80, P31913
[7]   The PI3K-mediated activation of CRAC independently regulates adenylyl cyclase activation and chemotaxis [J].
Corner, FI ;
Lippincott, CK ;
Masbad, JJ ;
Parent, CA .
CURRENT BIOLOGY, 2005, 15 (02) :134-139
[8]   Subsecond reorganization of the actin network in cell motility and chemotaxis [J].
Diez, S ;
Gerisch, G ;
Anderson, K ;
Müller-Taubenberger, A ;
Bretschneider, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (21) :7601-7606
[9]   CYCLIC 3',5'-AMP RELAY IN DICTYOSTELIUM-DISCOIDEUM .4. RECOVERY OF THE CAMP SIGNALING RESPONSE AFTER ADAPTATION TO CAMP [J].
DINAUER, MC ;
STECK, TL ;
DEVREOTES, PN .
JOURNAL OF CELL BIOLOGY, 1980, 86 (02) :545-553
[10]   CYCLIC 3',5'-AMP RELAY IN DICTYOSTELIUM-DISCOIDEUM .5. ADAPTATION OF THE CAMP SIGNALING RESPONSE DURING CAMP STIMULATION [J].
DINAUER, MC ;
STECK, TL ;
DEVREOTES, PN .
JOURNAL OF CELL BIOLOGY, 1980, 86 (02) :554-561