Detection of IgM to hepatitis B core antigen in a reductant containing, chemiluminescence assay

被引：11

作者：

Cheng, Y ^{[1
]}

Dubovoy, N ^{[1
]}

Hayes-Rogers, ME ^{[1
]}

Stewart, J ^{[1
]}

Shah, D ^{[1
]}

机构：

[1] Abbott Labs, Abbott Diagnost Div, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 230卷 / 1-2期

关键词：

IgM; RSF; PRISM; reductant;

D O I：

10.1016/S0022-1759(99)00128-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Abbott PRISM(R) hepatitis B core (HBc) antigen assay is an automatic in vitro competitive chemiluminescence immunoassay for the detection of total antibody to HBc (anti-HBc) antigen in human serum or plasma. The assay utilizes cysteine solution as a reducing reagent in order to maximize specificity. To help understand the effect of cysteine on detection of anti-HBc antigen, we separated and purified anti-HBc IgM and Ige from human plasma using size exclusion, protein A/G, and affinity chromatography techniques. We showed that cysteine affected the reactivity of anti-HBc IgM with recombinant HBc (rHBc) antigen but not the reactivity of anti-HBc:IgG, Anti-HBc IgM treated with cysteine yielded byproducts which were reactive in the PRISM HBcore assay. Reduction-sensitive factor (RSF) - IgM fraction from serum known to be non-specific for anti-HBc activity, similarly treated with cysteine, was no longer reactive in the PRISM HBcore assay. We showed that cysteine treatment is effective against non-specific IgM in human blood. Also, the inclusion of cysteine in the PRISM HBcore assay does not compromise the detection of HBc specific antibodies. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 35

页数：7

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