Adiponectin down-regulates acyl-coenzyme A:cholesterol acyltransferase-1 in cultured human monocyte-derived macrophages

被引：78

作者：

Furukawa, K

Hori, M

Ouchi, N

Kihara, S

Funahashi, T

Matsuzawa, Y

Miyazaki, A

Nakayama, H

Horiuchi, S ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Med & Pharmaceut Sci, Dept Med Biochem, Kumamoto, Japan

[2] Kumamoto Univ, Grad Sch Med & Pharmaceut Sci, Dept Molecular Cell Funct, Kumamoto, Japan

[3] Osaka Univ, Grad Sch Med, Dept Internal Med & Mol Sci, Osaka, Japan

[4] Showa Univ, Sch Med, Dept Biochem, Tokyo, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 317卷 / 03期

基金：

日本学术振兴会;

关键词：

adiponectin; acyl-coenzyme A : cholesterol acyltransferase-1; macrophages; foam cell; atherosclerosis;

D O I：

10.1016/j.bbrc.2004.03.123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acyl-coenzyrne A:cholesterol acyltransferase-1 (ACAT-1) catalyzes the formation of cholesteryl esters (CE) and plays a significant role in formation of macrophage-derived foam cells in atherosclerotic lesions. Adiponectin was reported to play an antiatherogenic role by inhibiting class A scavenger receptor (SR-A) expression in human macrophages. To further clarify its additional property, we examined its effect on ACAT-1 expression using human macrophages. Immunoblot analyses revealed a significant reduction of ACAT-1 protein by a low concentration (1 mug/ml) of adiponectin. The ACAT activity was also decreased in parallel by adiponectin. Northern blot analyses revealed that all four ACAT-1 mRNA transcripts (2.8, 3.6, 4.3, and 7.0 kb) were decreased almost equally by adiponectin. Furthermore, acetyl-LDL-induced CE-accumulation in these macrophages was reduced significantly by this adipocytokine. These results demonstrate the inhibitory effect of adiponectin on ACAT-1 expression, suggesting that adiponectin may play an anti-atherogenic role by down-regulating the expression of ACAT-1 as well as SR-A in human macrophages. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：831 / 836

页数：6

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